- high-resolution terminology - matching measurements at high-resolution
Oxygen calibration - DatLab
Description
O2 calibration
Calibration of the oxygen sensor is a prerequisite for obtaining valid measurements of respiration. Accurate calibration of the oxygen sensor depends on (1) equilibration of the incubation medium with air oxygen partial pressure at the temperature defined by the experimenter; (2) zero oxygen calibration; (3) high stability of the POS signal tested for sufficiently long periods of time; (4) linearity of signal output with oxygen pressure in the range between oxygen saturation and zero oxygen pressure; and (5) accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration. The standard oxygen calibration procedure is described below for high-resolution respirometry with the automatic calibration routine by DatLab.
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Reference: MiPNet06.03 POS-calibration-SOP
MiPNet19.18D_O2k-Series_G_and_DatLab_6:_Calibration
MiPNet12.20_O2k-calibration_tutorial
MitoPedia methods: Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab
