Pajuelo-Reguera 2011 Br J Nutr: Difference between revisions
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{{Publication | {{Publication | ||
|title=Pajuelo D, Quesada H, DΓaz S, FernΓ‘ndez-Iglesias A, Arola-Arnal A, BladΓ© C, SalvadΓ³ J, Arola L (2011) Chronic dietary supplementation of proanthocyanidins corrects the mitochondrial dysfunction of brown adipose tissue caused by diet-induced obesity in Wistar rats. Br | |title=Pajuelo-Reguera D, Quesada H, DΓaz S, FernΓ‘ndez-Iglesias A, Arola-Arnal A, BladΓ© C, SalvadΓ³ J, Arola L (2011) Chronic dietary supplementation of proanthocyanidins corrects the mitochondrial dysfunction of brown adipose tissue caused by diet-induced obesity in Wistar rats. Br J Nutr 28:1-9. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21733324 PIMD:21733324] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/21733324 PIMD:21733324] | ||
|authors=Pajuelo D, Quesada H, Diaz S, Fernandez-Iglesias A, Arola-Arnal A, Blade C, Salvado J, Arola L | |authors=Pajuelo-Reguera D, Quesada H, Diaz S, Fernandez-Iglesias A, Arola-Arnal A, Blade C, Salvado J, Arola L | ||
|year=2011 | |year=2011 | ||
|journal=Br | |journal=Br J Nutr | ||
|abstract=The present study aims to determine the effects of grape seed proanthocyanidin extract (GSPE) on brown adipose tissue (BAT) mitochondrial function in a state of obesity induced by diet. Wistar male rats were fed with a cafeteria diet (Cd) for 4 months; during the last 21 d, two groups were treated with doses of 25 and 50 mg GSPE/kg body weight. In the BAT, enzymatic activities of citrate synthase, cytochrome c oxidase (COX) and ATPase were determined and gene expression was analysed by real-time PCR. The mitochondrial function of BAT was determined in fresh mitochondria by high-resolution respirometry using both pyruvate and carnitine-palmitoyl-CoA as substrates. The results show that the Cd causes an important decrease in the gene expression of sirtuin 1, nuclear respiratory factor 1, isocitrate dehydrogenase 3Ξ³ and COX5Ξ± and, what is more telling, decreases the levels of mitochondrial respiration both with pyruvate and canitine-palmitoyl-CoA. Most of these parameters, which are indicative of mitochondrial dysfunction due to diet-induced obesity, are improved by chronic supplementation of GSPE. The beneficial effects caused by the administration of GSPE are exhibited as a protection against weight gain, in spite of the Cd the rats were fed. These data indicate that chronic consumption of a moderate dose of GSPE can correct an energy imbalance in a situation of diet-induced obesity, thereby improving the mitochondrial function and thermogenic capacity of the BAT. | |abstract=The present study aims to determine the effects of grape seed proanthocyanidin extract (GSPE) on brown adipose tissue (BAT) mitochondrial function in a state of obesity induced by diet. Wistar male rats were fed with a cafeteria diet (Cd) for 4 months; during the last 21 d, two groups were treated with doses of 25 and 50 mg GSPE/kg body weight. In the BAT, enzymatic activities of citrate synthase, cytochrome c oxidase (COX) and ATPase were determined and gene expression was analysed by real-time PCR. The mitochondrial function of BAT was determined in fresh mitochondria by high-resolution respirometry using both pyruvate and carnitine-palmitoyl-CoA as substrates. The results show that the Cd causes an important decrease in the gene expression of sirtuin 1, nuclear respiratory factor 1, isocitrate dehydrogenase 3Ξ³ and COX5Ξ± and, what is more telling, decreases the levels of mitochondrial respiration both with pyruvate and canitine-palmitoyl-CoA. Most of these parameters, which are indicative of mitochondrial dysfunction due to diet-induced obesity, are improved by chronic supplementation of GSPE. The beneficial effects caused by the administration of GSPE are exhibited as a protection against weight gain, in spite of the Cd the rats were fed. These data indicate that chronic consumption of a moderate dose of GSPE can correct an energy imbalance in a situation of diet-induced obesity, thereby improving the mitochondrial function and thermogenic capacity of the BAT. | ||
|keywords= | |keywords=Mitochondria, Polyphenols, Proanthocyanidins, Obesity, Brown adipose tissue, Oxidative phosphorylation | ||
|mipnetlab=ES_Tarragona_Arola L | |mipnetlab=ES_Tarragona_Arola L | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Exercise physiology;nutrition;life style | |||
|diseases=Obesity | |||
|organism=Rat | |||
|tissues=Fat | |||
|preparations=Isolated mitochondria | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=F, N | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 15:34, 13 November 2017
| Pajuelo-Reguera D, Quesada H, DΓaz S, FernΓ‘ndez-Iglesias A, Arola-Arnal A, BladΓ© C, SalvadΓ³ J, Arola L (2011) Chronic dietary supplementation of proanthocyanidins corrects the mitochondrial dysfunction of brown adipose tissue caused by diet-induced obesity in Wistar rats. Br J Nutr 28:1-9. |
Pajuelo-Reguera D, Quesada H, Diaz S, Fernandez-Iglesias A, Arola-Arnal A, Blade C, Salvado J, Arola L (2011) Br J Nutr
Abstract: The present study aims to determine the effects of grape seed proanthocyanidin extract (GSPE) on brown adipose tissue (BAT) mitochondrial function in a state of obesity induced by diet. Wistar male rats were fed with a cafeteria diet (Cd) for 4 months; during the last 21 d, two groups were treated with doses of 25 and 50 mg GSPE/kg body weight. In the BAT, enzymatic activities of citrate synthase, cytochrome c oxidase (COX) and ATPase were determined and gene expression was analysed by real-time PCR. The mitochondrial function of BAT was determined in fresh mitochondria by high-resolution respirometry using both pyruvate and carnitine-palmitoyl-CoA as substrates. The results show that the Cd causes an important decrease in the gene expression of sirtuin 1, nuclear respiratory factor 1, isocitrate dehydrogenase 3Ξ³ and COX5Ξ± and, what is more telling, decreases the levels of mitochondrial respiration both with pyruvate and canitine-palmitoyl-CoA. Most of these parameters, which are indicative of mitochondrial dysfunction due to diet-induced obesity, are improved by chronic supplementation of GSPE. The beneficial effects caused by the administration of GSPE are exhibited as a protection against weight gain, in spite of the Cd the rats were fed. These data indicate that chronic consumption of a moderate dose of GSPE can correct an energy imbalance in a situation of diet-induced obesity, thereby improving the mitochondrial function and thermogenic capacity of the BAT. β’ Keywords: Mitochondria, Polyphenols, Proanthocyanidins, Obesity, Brown adipose tissue, Oxidative phosphorylation
β’ O2k-Network Lab: ES_Tarragona_Arola L
Labels: MiParea: Respiration, Exercise physiology;nutrition;life style
Pathology: Obesity
Organism: Rat Tissue;cell: Fat Preparation: Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: F, N
HRR: Oxygraph-2k
