Difference between revisions of "Park 2010 Sensors and Actuators B"
(Created page with "{{Publication |title=Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditio...") |
|||
| Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions | |title=Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147: 263–269. | ||
|authors=Park J, Pak YK, Pak JJ | |info=[http\www.sciencedirect.com\science\article\pii\S0925400510002959 ScienceDirect] | ||
|authors=Park J, Pak YK, Pak JJ | |||
|year=2010 | |year=2010 | ||
|journal= | |journal=Sensors and Actuators B: Chemical | ||
|abstract=This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate | |abstract=This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate(OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical | ||
(OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical | This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical | ||
sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. | sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000µm<sup>2</sup> WE and a 20µm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated | ||
The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode | sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. | ||
(WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The | first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its | ||
fabricated sensor showed a sensitivity of 2.84 A/cm2M and a 90% response time of 4.9 s in an average of | |||
5 sensors when a 25, | |||
first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated | |||
sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the | |||
zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its | |||
result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. | result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. | ||
|keywords=electrochemistry, reservoir-type, oxygen sensor, cellular respiration, oxygen consumption rate | |keywords=electrochemistry, reservoir-type, oxygen sensor, cellular respiration, oxygen consumption rate | ||
| Line 22: | Line 18: | ||
|tissues=Skeletal Muscle | |tissues=Skeletal Muscle | ||
|preparations=Intact Cell; Cultured; Primary | |preparations=Intact Cell; Cultured; Primary | ||
|additional=L6 rat skeletal muscle | |||
}} | }} | ||
Revision as of 09:11, 5 September 2011
| Park J, Pak YK, Pak JJ (2010) A microfabricated reservoir-type oxygen sensor for measuring the real-time cellular oxygen consumption rate at various conditions. Sensors and Actuators B 147: 263–269. |
» [http\www.sciencedirect.com\science\article\pii\S0925400510002959 ScienceDirect]
Park J, Pak YK, Pak JJ (2010) Sensors and Actuators B: Chemical
Abstract: This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate(OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical This paper presents a microfabricated reservoir-type oxygen sensor, which can accurately measure the solubilized oxygen concentration in real time, in order to measure the cellular oxygen consumption rate (OCR) in a solution containing cells. The fabricated oxygen sensor is composed of three-parts: electrochemical sensing electrodes, an oxygen-permeable membrane, and a reservoir for storing the solution. The oxygen transport rate through the membrane and the oxygen reaction rate at the working electrode (WE) surface are the two dominant parameters in determining the sensitivity of the oxygen sensor. The fabricated sensor showed a sensitivity of 2.84 A/cm2M and a 90% response time of 4.9 s in an average of 5 sensors when a 25,000µm2 WE and a 20µm polydimethylsiloxane membrane were used. This is the first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. first report in which the fastest response time has been achieved for the oxygen sensor. The fabricated sensor showed the repeatability with 154.05±1.87 nA at the full-oxygen state and 2.77±1.0 nA at the zero-oxygen state. The fabricated sensor was used to measure the uncoupled OCR of the L6 cells, and its result of 3.69±0.30 was almost identical to the result of 3.70±0.26 obtained from a commercial system. • Keywords: electrochemistry, reservoir-type, oxygen sensor, cellular respiration, oxygen consumption rate
Labels:
Organism: Rat
Tissue;cell: Skeletal Muscle"Skeletal Muscle" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
L6 rat skeletal muscle
