Permeabilized tissue or cells: Difference between revisions
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|abbr=P<sub>tic</sub> | |abbr=P<sub>tic</sub>, P<sub>c</sub> | ||
|description='''Permeabilized tissue''' (see [[permeabilized muscle fibres]]) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | |description='''Permeabilized tissue''' (see [[permeabilized muscle fibres]]) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or [[permeabilization of plasma membrane|chemically]], for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the [[mitochondrial|mt]]-membranes. | ||
'''Permeabilized cells''' are, therefore, not any longer [[intact cells]] or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | '''Permeabilized cells''' (P<sub>c</sub>) are, therefore, not any longer [[intact cells]] or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells. | ||
|info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | |info=[[MiPNet11.05]]; [[O2k-Publications: Permeabilized cells]] | ||
|type=Method | |type=Method | ||
Revision as of 18:38, 19 January 2016
- high-resolution terminology - matching measurements at high-resolution
Permeabilized tissue or cells
Description
Permeabilized tissue (see permeabilized muscle fibres) or cells are mitochondrial preparations obtained by selectively permeabilizing the plasma membrane mechanically or chemically, for the exchange of soluble molecules between the cytosolic phase and external medium, without damaging the mt-membranes.
Permeabilized cells (Pc) are, therefore, not any longer intact cells or viable cells, since the intactness of cells implies the intactness of the plasma membrane, and any typical quantiative cell viability test (trypan blue etc) evaluating the intactness of the plasma membrane, yields a 100% negative result on fully permeabilized cells.
Abbreviation: Ptic, Pc
Reference: MiPNet11.05; O2k-Publications: Permeabilized cells
MitoPedia methods:
Respirometry
