Ponsot 2006 J Appl Physiol (1985)

Ponsot E, Dufour SP, Zoll J, Doutrelau S, N'Guessan B, Geny B, Hoppeler H, Lampert E, Mettauer B, Ventura-Clapier R, Richard R (2006) Exercise training in normobaric hypoxia in endurance runners. II. Improvement of mitochondrial properties in skeletal muscle. J Appl Physiol (1985) 100:1249-57.

» PMID: 16339351 Open Access

Ponsot E, Dufour SP, Zoll J, Doutrelau S, N'Guessan B, Geny B, Hoppeler H, Lampert E, Mettauer B, Ventura-Clapier R, Richard R (2006) J Appl Physiol (1985)

Abstract: This study investigates whether adaptations of mitochondrial function accompany the improvement of endurance performance capacity observed in well-trained athletes after an intermittent hypoxic training program. Fifteen endurance-trained athletes performed two weekly training sessions on treadmill at the velocity associated with the second ventilatory threshold (VT2) with inspired O₂ fraction = 14.5 % [hypoxic group (Hyp), N = 8] or with inspired O² fraction = 21 % [normoxic group (Nor), N = 7], integrated into their usual training, for 6 wk. Before and after training, oxygen uptake (V_O2) and speed at VT2, maximal V_O2 (V_O2max), and time to exhaustion at velocity of V_O2max (minimal speed associated with V_O2max) were measured, and muscle biopsies of vastus lateralis were harvested. Muscle oxidative capacities and sensitivity of mitochondrial respiration to ADP (K_m) were evaluated on permeabilized muscle fibers. Time to exhaustion, V_O2 at VT2, and V_O2max were significantly improved in Hyp (+42, +8, and +5 %, respectively) but not in Nor. No increase in muscle oxidative capacity was obtained with either training protocol. However, mitochondrial regulation shifted to a more oxidative profile in Hyp only as shown by the increased K_m for ADP (Nor: before 476 +/- 63, after 524 +/- 62 microM, not significant; Hyp: before 441 +/- 59, after 694 +/- 51 microM, P < 0.05). Thus including hypoxia sessions into the usual training of athletes qualitatively ameliorates mitochondrial function by increasing the respiratory control by creatine, providing a tighter integration between ATP demand and supply.

• Bioblast editor: Gnaiger E

MitoEAGLE V_O2max/BME data base

• Human vastus lateralis
• 15 (8+7) males
• 30.6 years
• Athletic, highly trained distance runners; control groups combined
• H = 1.81 m
• M = 71.1 kg
• BME = 0.04
• BMI = 21.8 kg·m^-2
• V_O2max/M = 62.9 mL·min^-1·kg^-1
• Permeabilized muscle fibres; 22 °C; GM_P; m_d; conversions: Gnaiger 2009 Int J Biochem Cell Biol
• J_O2,P(NS) = 181 µmol·s^-1·kg^-1 wet muscle mass (37 °C)

• J_O2,P(GM) = 132 µmol·s^-1·kg^-1 wet muscle mass (37 °C)
• J_O2,P(NS) = J_O2,P(GM)/0.73
• Fiber wet mass to dry mass ratio = 3.5 (N'Guessan 2004 Mol Cell Biochem)

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References: BME and VO2max

» VO2max

	Reference
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Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Stress:Hypoxia  Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Intact organism 

Regulation: ADP  Coupling state: OXPHOS  Pathway: N 

VO2max, BMI, BME, MitoEAGLE BME