Racker 1974 J Biol Chem
| Racker E, Stoeckenius W (1974) Reconstitution of purple membrane vesicles catalyzing light-driven proton uptake and adenosine triphosphate formation. J Biol Chem 249: 662-663. |
Racker E, Stoeckenius W (1974) J Biol Chem
Abstract: The purple membrane from Halobacterium halobium was incorporated into phospholipid vesicles. On illumination (2 X 106 ergs per cm2 per s) the reconstituted vesicles took up protons (50 to 200 ng of ions H+ per mg of purple protein) which were released in the dark. Addition of valinomycin accelerated both the rate of uptake in the light and the release of protons in the dark. Uncouplers of oxidative phosphorylation abolished the uptake of protons. Inclusion of the mitochondrial oligomycin-sensitive ATPase during reconstitution yielded vesicles which catalyzed light-dependent phosphorylation. These reconstituted vesicles represent a simple model system for a biological proton pump capable of generating ATP from ADP and Pi. • Keywords: Halobacterium halobium, purple membrane vesicles, proton uptake, ATP, light
Labels:
Organism: Bacteria"Bacteria" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. Enzyme: Complex V; ATP Synthase"Complex V; ATP Synthase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Made history
