Rauchova 2005 Biochem Biophys Res Comm: Difference between revisions
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{{Publication | {{Publication | ||
|title=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339: 362- | |title=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339:362-6. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16300743 PMID: 16300743] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/16300743 PMID: 16300743] | ||
|authors=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z | |authors=Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z | ||
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|abstract=The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogsβidebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ<sub>1</sub>Β were found to be more efficient in the scavenging activity (IC<sub>50</sub> : 0.052 and 0.075 ΞΌM, respectively) than CoQ<sub>3</sub> (IC<sub>50</sub> : 45.8 ΞΌM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation | |abstract=The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogsβidebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ<sub>1</sub>Β were found to be more efficient in the scavenging activity (IC<sub>50</sub> : 0.052 and 0.075 ΞΌM, respectively) than CoQ<sub>3</sub> (IC<sub>50</sub> : 45.8 ΞΌM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation | ||
|keywords=Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs | |keywords=Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs | ||
|mipnetlab=CZ Prague Houstek J, IT Bologna Lenaz G, IT Bologna Genova ML | |||
|discipline=Pharmacology; Biotechnology | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|injuries=Oxidative stress;RONS | |||
|pathways=S, Gp, Other combinations | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Pharmacology; Biotechnology | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
Latest revision as of 13:53, 27 March 2018
| Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Inhibition of glycerophosphate-dependent H2O2 generation in brown fat mitochondria by idebenone. Biochem Biophys Res Comm 339:362-6. |
Rauchova H, Vrbacky M, Bergamini C, Fato R, Lenaz G, Houstek J, Drahota Z (2005) Biochem. Biophys. Res. Commun.
Abstract: The established protective effect of coenzyme Q (CoQ) analogs is dependent on the location of reactive oxygen species (ROS) generation. One of these analogsβidebenone (hydroxydecyl-ubiquinone) is used as an antioxidative therapeutic drug. We tested its scavenging effect on the glycerophosphate (GP)-dependent ROS production as this enzyme was shown as a new site in the mitochondrial respiratory chain where ROS can be generated. We observed that idebenone inhibits both GP- and succinate-dependent ROS production. Idebenone and CoQ1 were found to be more efficient in the scavenging activity (IC50 : 0.052 and 0.075 ΞΌM, respectively) than CoQ3 (IC50 : 45.8 ΞΌM). Idebenone also inhibited ferricyanide (FeCN)-activated, GP-dependent ROS production. Our data thus extend previous findings on the scavenging effect of idebenone and show that it can also eliminate GP-dependent ROS generation β’ Keywords: Mitochondrial glycerophosphate dehydrogenase, Succinate dehydrogenase, Reactive oxygen species, debenone, Coenzyme Q analogs
β’ O2k-Network Lab: CZ Prague Houstek J, IT Bologna Lenaz G, IT Bologna Genova ML
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS
Pathway: S, Gp, Other combinations
HRR: Oxygraph-2k
