Difference between revisions of "Ruthenium red"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Rut | |abbr=Rut | ||
|description='''Ruthenium red''' (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca<sup>2+</sup> uniporter. | |description='''Ruthenium red''' (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca<sup>2+</sup> uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca<sup>2+</sup>-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2]. | ||
|info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | |info=[[MiPNet03.02]], [http://en.wikipedia.org/wiki/Ruthenium_red Wikipedia] | ||
}} | }} | ||
{{MitoPedia methods}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
== References == | |||
[1] Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856. | |||
[2] Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204. | |||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 10:47, 13 December 2013
- high-resolution terminology - matching measurements at high-resolution
Ruthenium red
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: Rut
Reference: MiPNet03.02, Wikipedia
MitoPedia topics: Inhibitor
References
[1] Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
[2] Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
Application in HRR
Rut: Ruthenium Red (technical grade; [(NH3)5RuORu(NH3)4ORu(NH3)5]Cl6); Sigma R 2751, 1 g; FW = 786.35
Preparation of 10 mM stock solution (dissolved in H2O):
- 1) weigh 7.86 mg of Ruthenium Red
- 2) dissolve in 1 ml H2O
- 3) divide into 0.2 ml aliquots
- 4) store frozen at -20 °C
