- high-resolution terminology - matching measurements at high-resolution
Ruthenium red
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: Rut
Reference: MiPNet03.02, Wikipedia
MitoPedia topics: Inhibitor
Application in HRFR
Rut: Ruthenium Red (technical grade; [(NH3)5RuORu(NH3)4ORu(NH3)5]Cl6); Sigma R 2751, 1 g; FW = 786.35.
Preparation of 10 mM stock solution (dissolved in H2O):
- 1) Weigh 7.86 mg Ruthenium Red.
- 2) Dissolve in 1 ml H2O.
- 3) Divide into 0.2 ml aliquots.
- 4) Store at -20 °C.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
