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Difference between revisions of "SUIT protocol library"

From Bioblast
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'''''This page is in preparation'''''
'''''This page is in preparation''''' (intern wiki: Library of protocols)


= Classes of protocols with coupling and substrate control =
= Classes of protocols with coupling and substrate control =
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# '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional)
# '''Diametral coupling -> substrate ->> control''' (mono- or bidirectional)
# '''Diametral substrate -> coupling ->> control''' (mono- or bidirectional)
# '''Diametral substrate -> coupling ->> control''' (mono- or bidirectional)
=== CI -> CI+CII -> CII ===
Respiratory states: CI<sub>L</sub> -> CI<sub>P</sub> -> CI+II<sub>P</sub> -> CI+II<sub>E</sub> -> CII<sub>E</sub> -> ROX
* '''GM+D+S+F+Rot''' ''Innsbruck-Copenhagen protocol'': GM+D+c+S+F+Rot
::Cyt c: added after ADP; cyt c added after S; ADP conc: _ _; ROX: Ama ([[Boushel 2007 Diabetologia]])
* '''PMG+D+S+F+Rot''' ''Brain protocol'': PMG+D+c+S+F+Rot
::ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the [[OXPHOS]] state (CI+CII) is saturating ([[Sumbalova 2011 Abstract Bordeaux]]); [[ROX]]: Mna+Ama; Myx
=== CI -> CI -> CI+CII -> CII ===
* '''GM+D+P+S+F+Rot''' ''Innsbruck-Liege protocol 1'': GM+D+c+P+S+F+Rot
::[[Cytochrome c]]: early addition of ''c'' ensures comparability of all states in case of any ''c'' effect.
* '''PM+D+G+S+F+Rot''' ''Innsbruck-Liege protocol 2'': PM+D+c+G+S+F+Rot
=== ETF -> CI -> CI+CII -> CII ===
Respiratory states: ETF<sub>L</sub> -> ETF<sub>P</sub> -> CI<sub>P</sub> -> CI+II<sub>P</sub> -> CI+II<sub>E</sub> -> CII<sub>E</sub> -> ROX
* '''OctM+D+G+S+F+Rot''' ''Innsbruck training protocol'': OctM+D+G+c+S+F+Rot
::[[Pesta 2012 Methods Mol Biol]], [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]]
Respiratory states: ETF<sub>L</sub> -> ETF<sub>P</sub> -> CI<sub>P</sub> -> CI+II<sub>P</sub> -> CII<sub>P</sub> -> CII<sub>L</sub> -> CII<sub>E</sub> -> ROX
* '''OctM+D+G+S+Rot+Omy+F''' ''Greenland-Monte Rosa protocol'': OctM+D+G+S+Rot+Omy+F+c
::[[Cytochrome c|Cyt ''c'']]: added after F. The absence of a significant ''c''-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k ([[MiR05]]); Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation ([[Pesta 2012 Methods Mol Biol]]); [[ROX]]: Mna+Ama
= Experimental details =
== Oxygen saturation ==
== ADP kinetics ==
== Cytochrome c ==
[[#CI -> CI+CII -> CII|CI -> CI+CII -> CII]]
[[#ETF -> CI -> CI+CII -> CII|ETF -> CI -> CI+CII -> CII]]
== ROX ==




Revision as of 14:18, 18 April 2012

This page is in preparation (intern wiki: Library of protocols)

Classes of protocols with coupling and substrate control

Linear

  1. Linear coupling control: at a single substrate state (classical State 3 -> State 4).
  2. Linear substrate control: at a single coupling state (unusual).
  3. Monodirectional linear coupling control: LN -> PD -> EF
  4. Bidirectional linear coupling control: LN -> PD -> LOmy -> EF
  • CI
  • CII
  • CI+CII
  • CIV
  • ETF
  • GPDH


Orthogonal

  1. Orthogonal coupling -> substrate control (mono- or bidirectional)
  2. Orthogonal substrate -> coupling control (mono- or bidirectional)


Diametral

  1. Diametral coupling -> substrate ->> control (mono- or bidirectional)
  2. Diametral substrate -> coupling ->> control (mono- or bidirectional)


References and Notes

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