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SUIT reference protocol

From Bioblast


high-resolution terminology - matching measurements at high-resolution


SUIT reference protocol

Description

The SUIT reference assay consists of two SUIT reference protocols (SUIT-RP1 and SUIT-RP2) which are harmonized SUIT protocols. These are coordinated such that they can be statistically evaluated as replicate measurements of cross-linked respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, the two SUIT-RP are complementary with their focus on specific respiratory coupling and substrate control aspects, extending previous strategies for respirometrc OXPHOS analysis.

SUIT-RP1: PM(LPcE)+Oct+G+S+Rot+Gp+Ama+Tm+Azd
SUIT-RP1: 1PM(L2Pc3E)_4Oct_5G_6S_7Rot_8Gp_9Ama_10Tm_11Azd
SUIT-RP2: D(ROX)+Oct(P)+M+Pc+G+S(PE)+Gp+Rot+Ama+Tm+Azd
SUIT-RP2: 1D(ROX)_2Oct(P)_3M_4Pc_5G_6S(P7E)_8Gp_9Rot_10Ama_11Tm_12Azd

Abbreviation: SUIT-RP

Reference: MiPNet21.06 SUIT reference assay



Template:MitoPedia SUIT


Cross-linked respiratory states

The cross-linked respiratory states for harmonization between protocols are:


SUIT RP1


SUIT RP2