Schneeberger 2008 Shock

[[Has title::Schneeberger S, Kuznetsov AV, Seiler R, Renz O, Meusburger H, Mark W, Brandacher G, Margreiter R, Gnaiger E (2008) Mitochondrial ischemia-reperfusion injury of the transplanted rat heart: improved protection by preservation versus cardioplegic solutions. Shock 30: 365-371.]]

» [[Has info::PMID: 18317412]]

Was written by::Schneeberger S, Was written by::Kuznetsov AV, Was written by::Seiler R, Was written by::Renz O, Was written by::Meusburger H, Was written by::Mark W, Was written by::Brandacher G, Was written by::Margreiter R, Was written by::Gnaiger E (Was published in year::2008) Was published in journal::Shock

Abstract: [[has abstract::Cold ischemia time and preservation of organs are limited by I/R injury leading to primary nonfunction of the graft. In a rat heart transplant model, we compared cardioplegic St Thomas (ST) to histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin preservation solutions in terms of contractile function, and mitochondrial respiratory and enzymatic defects after prolonged cold ischemia and reperfusion. Contractile function was scored after transplantation and 24 h of reperfusion. Mitochondrial function was investigated by high-resolution respirometry of permeabilized myocardial fibers. Graft performance in terms of contractile function declined with the duration of cold storage. Recovery was significantly improved after 10 h of cold storage in HTK compared with ST (cardiac scores, 3.3 ± 0.5 and 1.8 ± 0.8, respectively). Tissue lactate dehydrogenase was better preserved in HTK than ST. Increase of tissue water content(edema) was less pronounced in HTK than ST (3.33 ± 0.14 and 3.73 ± 0.21 mg/mg dry weight, respectively). Similar cardiac scores (2.6 ± 0.9 and 2.9 ± 1.2, respectively) and mitochondrial respiratory parameters were obtained after preservation in HTK and University of Wisconsin. Decline in contractile function of individual grafts correlated well with loss of mitochondrial respiratory capacity, whereas citrate synthase activity remained largely preserved, indicating specific damage of respiratory complexes. Our data provide evidence for the superiority of preservation solutions versus a cardioplegic solution for prolonged cold storage of the heart. The correlation of graft performance and mitochondrial function indicates the potential of high-resolution respirometry for quantitative assessment of myocardial injury upon cold I/R, providing a basis for diagnostic approaches and evaluation of improved preservation solutions for heart transplantation.]] • Keywords: has publicationkeywords::Heart preservation, has publicationkeywords::Heart transplantation, has publicationkeywords::Cardiac function, has publicationkeywords::Mitochondrial function, has publicationkeywords::Permeabilized fibers

• O2k-Network Lab: Was published by MiPNetLab::AT_Innsbruck_Gnaiger E, Was published by MiPNetLab::AT Innsbruck MitoCom

Labels:

Stress:Injury and adaptation::Ischemia-Reperfusion; Preservation  Organism: Organism::Rat  Tissue;cell: tissue and cell::Heart  Preparation: Preparation::Intact Organ, Preparation::Permeabilized tissue, Preparation::Enzyme 

Regulation: Topic::Substrate; Glucose; TCA Cycle  Coupling state: Coupling states::OXPHOS 

HRR: Instrument and method::Oxygraph-2k