Schneider 1950 J Biol Chem

Schneider WC, Hogeboom GH (1950) Intracellular distribution of enzymes V. Further studies on the distribution of cytochrome c in rat liver homogenates.. J Biol Chem 183: 123-128.

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Schneider WC, Hogeboom GH (1950) J Biol Chem

Abstract:

1. The cytochrome c content and the succinoxidase activity of fractions isolated by means of differential centrifugation from homogenates of rat liver in 0.88 M sucrose and in 0.25 M sucrose were determined.
2. Approximately 50 per cent of the total cytochrome c and 60 per cent of the total succinoxidase activity (determined in the presence of excess added cytochrome c) of the rat liver homogenates were recovered in the mitochondrial fraction isolated in either 0.88 M or 0.25 M sucrose. The biological activity of the cytochrome c present in the mitochondrial fractions was demonstrated by measurement of the succinoxidase activity of the mitochondria in the absence of added cytochrome c.

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