Schneider 1950 J Biol Chem
| Schneider WC, Hogeboom GH (1950) Intracellular distribution of enzymes V. Further studies on the distribution of cytochrome c in rat liver homogenates. J Biol Chem 183: 123-128. |
Schneider WC, Hogeboom GH (1950) J Biol Chem
Abstract:
- The cytochrome c content and the succinoxidase activity of fractions isolated by means of differential centrifugation from homogenates of rat liver in 0.88 M sucrose and in 0.25 M sucrose were determined.
- Approximately 50 per cent of the total cytochrome c and 60 per cent of the total succinoxidase activity (determined in the presence of excess added cytochrome c) of the rat liver homogenates were recovered in the mitochondrial fraction isolated in either 0.88 M or 0.25 M sucrose. The biological activity of the cytochrome c present in the mitochondrial fractions was demonstrated by measurement of the succinoxidase activity of the mitochondria in the absence of added cytochrome c.
• Keywords: cytochrome c distribution, rat liver, succinoxidase activity
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
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