Difference between revisions of "Schoenfeld 2010 Biochim Biophys Acta"
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{{Publication | {{Publication | ||
|title= | |title=Schönfeld P, Wieckowski MR, Lebiedzinska M, Wojtczak L (2010) Mitochondrial fatty acid oxidation and oxidative stress: Lack of reverse electron transfer-associated production of reactive oxygen species. Biochim Biophys Acta 1797:929-38. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20085746 PMID: 20085746 Open Access] | |||
|authors=Schoenfeld P, Wieckowski MR, Lebiedzinska M, Wojtczak L | |authors=Schoenfeld P, Wieckowski MR, Lebiedzinska M, Wojtczak L | ||
|year=2010 | |year=2010 | ||
|journal=Biochim | |journal=Biochim Biophys Acta | ||
|abstract=Reverse electron transfer (RET) from succinate to NAD<sup>+</sup> is known to be accompanied by high generation of reactive oxygen species (ROS). In contrast, oxidation of fatty acids by mitochondria, despite being a powerful source of FADH<sub>2</sub>, does not lead to RET-associated high ROS generation. Here we show that oxidation of carnitine esters of medium- and long-chain fatty acids by rat heart mitochondria is accompanied by neither high level of NADH/NAD<sup>+</sup> nor intramitochondrial reduction of acetoacetate to beta-hydroxybutyrate, comparable to those accompanying succinate oxidation, although it produces the same or higher energization of mitochondria as evidenced by high transmembrane potential. Also in contrast to the oxidation of succinate, where conversion of the pH difference between the mitochondrial matrix and the medium into the transmembrane electric potential by addition of nigericin results in a decrease of ROS generation, the same treatment during oxidation of octanoylcarnitine produces a large increase of ROS. Analysis of respiratory chain complexes by Blue Native polyacrylamide gel electrophoresis revealed bands that could tentatively point to supercomplex formation between complexes II and I and complexes II and III. However, no such association could be found between complex I and the electron transferring flavoprotein that participates in fatty acid oxidation. It is speculated that structural association between respective respiratory chain components may facilitate effective reverse electron transfer. | |||
|abstract=Reverse electron transfer (RET) from succinate to NAD | |keywords=Fatty acid oxidation, Reverse electron transfer, Reactive oxygen species (ROS), Mitochondrion, Respiratory chain complex | ||
|keywords=Fatty acid oxidation | |mipnetlab=DE Magdeburg Siemen D, DE Magdeburg Schoenfeld P | ||
| | |discipline=Mitochondrial Physiology | ||
|articletype=Protocol; Manual | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|injuries=Oxidative stress;RONS | |||
|organism=Rat | |||
|tissues=Heart | |||
|preparations=Isolated mitochondria | |||
|enzymes=Complex I, Complex II;succinate dehydrogenase, Supercomplex | |||
|topics=Redox state, Fatty acid | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
| | |articletype=Protocol; Manual | ||
}} | }} | ||
* [[Oroboros Instruments |Bioenergetics and Biomedical Instruments]] | |||
Latest revision as of 17:17, 15 February 2023
| Schönfeld P, Wieckowski MR, Lebiedzinska M, Wojtczak L (2010) Mitochondrial fatty acid oxidation and oxidative stress: Lack of reverse electron transfer-associated production of reactive oxygen species. Biochim Biophys Acta 1797:929-38. |
Schoenfeld P, Wieckowski MR, Lebiedzinska M, Wojtczak L (2010) Biochim Biophys Acta
Abstract: Reverse electron transfer (RET) from succinate to NAD+ is known to be accompanied by high generation of reactive oxygen species (ROS). In contrast, oxidation of fatty acids by mitochondria, despite being a powerful source of FADH2, does not lead to RET-associated high ROS generation. Here we show that oxidation of carnitine esters of medium- and long-chain fatty acids by rat heart mitochondria is accompanied by neither high level of NADH/NAD+ nor intramitochondrial reduction of acetoacetate to beta-hydroxybutyrate, comparable to those accompanying succinate oxidation, although it produces the same or higher energization of mitochondria as evidenced by high transmembrane potential. Also in contrast to the oxidation of succinate, where conversion of the pH difference between the mitochondrial matrix and the medium into the transmembrane electric potential by addition of nigericin results in a decrease of ROS generation, the same treatment during oxidation of octanoylcarnitine produces a large increase of ROS. Analysis of respiratory chain complexes by Blue Native polyacrylamide gel electrophoresis revealed bands that could tentatively point to supercomplex formation between complexes II and I and complexes II and III. However, no such association could be found between complex I and the electron transferring flavoprotein that participates in fatty acid oxidation. It is speculated that structural association between respective respiratory chain components may facilitate effective reverse electron transfer. • Keywords: Fatty acid oxidation, Reverse electron transfer, Reactive oxygen species (ROS), Mitochondrion, Respiratory chain complex
• O2k-Network Lab: DE Magdeburg Siemen D, DE Magdeburg Schoenfeld P
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart Preparation: Isolated mitochondria Enzyme: Complex I, Complex II;succinate dehydrogenase, Supercomplex Regulation: Redox state, Fatty acid Coupling state: OXPHOS
HRR: Oxygraph-2k
