Sharaf 2015 Abstract IOC106: Difference between revisions
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|abstract=Zinc and calcium have highly interwoven functions that are essential for cellular homeostasis. Here, we studied the mechanisms of their import into mitochondria and interactions on oxidative phosphorylation (OXPHOS) and membrane potential (∆Ψm). We showed that while the two cations permeated the mitochondrial inner membrane via different mechanisms they synergistically inhibited OXPHOS but antagonistically dissipated ∆Ψm. Ruthenium red completely prevented calcium-induced OXPHOS inhibition suggesting that exclusive entry of calcium into the matrix via mitochondrial calcium uniporter is a fundamental step for the functional impairment caused by this cation. Overall, these data indicate that interactions of zinc and calcium on mitochondrial function result from mechanisms other than competition for their uptake pathways. | |abstract=Zinc and calcium have highly interwoven functions that are essential for cellular homeostasis. Here, we studied the mechanisms of their import into mitochondria and interactions on oxidative phosphorylation (OXPHOS) and membrane potential (∆Ψm). We showed that while the two cations permeated the mitochondrial inner membrane via different mechanisms they synergistically inhibited OXPHOS but antagonistically dissipated ∆Ψm. Ruthenium red completely prevented calcium-induced OXPHOS inhibition suggesting that exclusive entry of calcium into the matrix via mitochondrial calcium uniporter is a fundamental step for the functional impairment caused by this cation. Overall, these data indicate that interactions of zinc and calcium on mitochondrial function result from mechanisms other than competition for their uptake pathways. | ||
|keywords=Zinc, Calcium, Mitochondria, Mitochondrial respiration, Mitochondrial membrane potential, MCU | |keywords=Zinc, Calcium, Mitochondria, Mitochondrial respiration, Mitochondrial membrane potential, MCU | ||
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==Affiliations and Support== | ==Affiliations and Support== | ||
Department of Biomedical Sciences, | Department of Biomedical Sciences, | ||
Revision as of 13:31, 24 September 2015
| Sharaf MS, Van den Heuvel MR, Stevens D, Kamunde C (2015) Mechanisms of mitochondrial import and interactions of zinc and calcium. Mitochondr Physiol Network 20.10. |
Link:
Sharaf MS, Van den Heuvel MR, Stevens D, Kamunde C (2015)
Event: IOC106 Schroecken
Zinc and calcium have highly interwoven functions that are essential for cellular homeostasis. Here, we studied the mechanisms of their import into mitochondria and interactions on oxidative phosphorylation (OXPHOS) and membrane potential (∆Ψm). We showed that while the two cations permeated the mitochondrial inner membrane via different mechanisms they synergistically inhibited OXPHOS but antagonistically dissipated ∆Ψm. Ruthenium red completely prevented calcium-induced OXPHOS inhibition suggesting that exclusive entry of calcium into the matrix via mitochondrial calcium uniporter is a fundamental step for the functional impairment caused by this cation. Overall, these data indicate that interactions of zinc and calcium on mitochondrial function result from mechanisms other than competition for their uptake pathways.
• Keywords: Zinc, Calcium, Mitochondria, Mitochondrial respiration, Mitochondrial membrane potential, MCU
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Affiliations and Support
Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada C1A 4P3
