Difference between revisions of "Singh 2013 J Cereb Blood Flow Metab"
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{{Publication | {{Publication | ||
|title=Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED (2013) Phenelzine mitochondrial functional preservation and neuroprotection after traumatic brain injury related to scavenging of the lipid peroxidation-derived aldehyde 4-hydroxy-2-nonenal. J Cereb Blood Flow Metab 33: 593- | |title=Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED (2013) Phenelzine mitochondrial functional preservation and neuroprotection after traumatic brain injury related to scavenging of the lipid peroxidation-derived aldehyde 4-hydroxy-2-nonenal. J Cereb Blood Flow Metab 33:593-9 | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/23321786 PMID: 23321786] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/23321786 PMID: 23321786] | ||
|authors=Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED | |authors=Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |organism=Rat | ||
|tissues=Nervous system | |tissues=Nervous system | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex I, Complex II;succinate dehydrogenase | |||
|topics=Fatty acid | |||
|substratestates=CI, CII | |substratestates=CI, CII | ||
| | |instruments=Oxygraph-2k | ||
}} | }} |
Revision as of 10:59, 13 February 2015
Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED (2013) Phenelzine mitochondrial functional preservation and neuroprotection after traumatic brain injury related to scavenging of the lipid peroxidation-derived aldehyde 4-hydroxy-2-nonenal. J Cereb Blood Flow Metab 33:593-9 |
Singh IN, Gilmer LK, Miller DM, Cebak JE, Wang JA, Hall ED (2013) J Cereb Blood Flow Metab
Abstract: Phenelzine (PZ) is a scavenger of the lipid peroxidation (LP)-derived reactive aldehyde 4-hydroxynonenal (4-HNE) due to its hydrazine functional group, which can covalently react with 4-HNE. In this study, we first examined the ability of PZ to prevent the respiratory depressant effects of 4-HNE on normal isolated brain cortical mitochondria. Second, in rats subjected to controlled cortical impact traumatic brain injury (CCI-TBI), we evaluated PZ (10โmg/kg subcutaneously at 15โminutes after CCI-TBI) to attenuate 3-hour post-TBI mitochondrial respiratory dysfunction, and in separate animals, to improve cortical tissue sparing at 14 days. While 4-HNE exposure inhibited mitochondrial complex I and II respiration in a concentration-dependent manner, pretreatment with equimolar concentrations of PZ antagonized these effects. Western blot analysis demonstrated a PZ decrease in 4-HNE in mitochondrial proteins. Mitochondria isolated from peri-contusional brain tissue of CCI-TBI rats treated with vehicle at 15โminutes after injury showed a 37% decrease in the respiratory control ratio (RCR) relative to noninjured mitochondria. In PZ-treated rats, RCR suppression was prevented (P<0.05 versus vehicle). In another cohort, PZ administration increased spared cortical tissue from 86% to 97% (P<0.03). These results suggest that PZ's neuroprotective effect is due to mitochondrial protection by scavenging of LP-derived 4-HNE. โข Keywords: Phenelzine, Traumatic brain injury, Mitochondrial respiratory dysfunction
Labels:
Organism: Rat
Tissue;cell: Nervous system
Preparation: Isolated mitochondria
Enzyme: Complex I, Complex II;succinate dehydrogenase
Regulation: Fatty acid
HRR: Oxygraph-2k