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| {{MitoPedia
| | #REDIRECT [[O2k-Fluo Smart-Module]] |
| |description='''Smart Fluo-Sensor''': LED with specified wavelength ([[Smart Fluo-Sensor Green]] and [[Smart Fluo-Sensor Blue]]), photodiode, [[Filter-Cap]] attached with specific optical filter for the LED and photodiode. Smart Fluo-Sensors are precalibrated with sensor-specific memory and direct input into DatLab 7.
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| |info=[[O2k-FluoRespirometer]]
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| }}
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| {{MitoPedia concepts}}
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| {{MitoPedia methods
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| |mitopedia method=Fluorometry
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| }}
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| {{MitoPedia O2k and high-resolution respirometry
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| |mitopedia O2k and high-resolution respirometry=O2k hardware
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| }}
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| {{MitoPedia topics}}
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| [[File:Smart_Fluo-Sensor_-_connect_.png|thumb|150px|'''Orientation''' for connection]]
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| [[File:Smart_Fluo-Sensor_RIGHT.jpg|thumb|150px|'''OK''', fully inserted]]
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| [[File:Smart_Fluo-Sensor_WRONG.jpg|thumb|150px|'''WRONG''', not fully inserted]] | |
| [[File:Smart_Fluo-Sensor_inserted.jpg|thumb|150px|'''Horizontal''' cable routing]]
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| __TOC__
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| {{Template:Technical support integrated}}
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| == O2k-Guide ==
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| === Select the Smart Fluo-Sensors ===
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| :::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
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| :::: Select the Fluo-Sensor and filter set form the table on [[MiPNet17.05_O2k-Fluorescence_LED2-Module#Fluorophores|application-specific settings]]. Each Fluo-Sensor is delivered with a mounted filter set:
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| ::::# [[Fluorescence-Sensor Green]]: 525 nm. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]].
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| ::::# [[Fluorescence-Sensor Blue]]: 465 nm. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green®.
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| '''O2k-Guide: Setup - next step [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''
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| === Connect Smart Fluo-Sensor to O2k ===
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| :::: Connect the Smart Fluo-Sensor cable to the Fluo plug of the O2k, insert the male plug of the cable into the female Fluo plug. The red dot on the male plug faces straight upwards. Each Smart Fluo-Sensor can be used on O2k-Chamber A or B. The blue frame of the chamber window and the Smart Fluo-Sensor are specially designed to align in a specific rotational position with the cable extending horizontally to left (chamber A) and right (chamber B). The Smart Fluo-Sensor is carefully inserted into the window frame and rotated into final position, leaving no gap between windon frame and sensor body.
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| :::: To remove the Smart Fluo-Sensor, carefully pull out the sensor body with slight back and forth rotations. Do not pull on the cable.
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| === Stoppers ===
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| :::: Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. The black stoppers can be used for all HRFR applications.
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| <!-- '''O2k-Guide: [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings]]''' -->
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| [[Category:Technical service]]
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