Difference between revisions of "Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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{{Abstract | {{Abstract | ||
|title=Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | |title=Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum] | |info=[http://www.bioblast.at/index.php/Talk:Fatty_acid_oxidation O2k-Network Discussion Forum] | ||
|authors=Smith CD | |authors=Smith CD | ||
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|abstract=I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine. ย | |abstract=I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine. ย | ||
'''Protocol''': For maximal respiration we make sequential additions of 18 ยตM PC, 20 ยตM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. | '''Protocol''': For maximal respiration we make sequential additions of 18 ยตM PC, 20 ยตM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition. | ||
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{{Labeling | {{Labeling | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates= | |couplingstates=OXPHOS, ET | ||
| | |pathways=F | ||
|additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | |additional=Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine | ||
}} | }} |
Latest revision as of 16:08, 13 November 2017
Smith 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Smith CD (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
I have extensively tested FAO in PMFBs using the Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine.
Protocol: For maximal respiration we make sequential additions of 18 ยตM PC, 20 ยตM PCoA, and 5 mM Carnitine. Addition of ADP induces a peak rate of O2 consumption that is not increased with FCCP addition.
Labels:
Preparation: Permeabilized tissue
Coupling state: OXPHOS, ET
Pathway: F
Palmitoylcarnitine, Palmitoyl-CoA, and L-Carnitine