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Sodium phosphate buffer

From Bioblast


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Sodium phosphate buffer

Description

Sodium phosphate buffer, Na-PB, for HRR with freeze-dried bakerยดs yeast.

Abbreviation: Na-PB






MitoPedia topics: Media for respirometry 

Application in HRFR

  • experimental temperature = 28ยฐC
  • oxygen solubility factor of Na-PB = 0.95


Na-PB (50 mM, pH 7.1)

Dissolve 3.9 g NaH2PO4 x 2 H2O (Sigma 71505, FW = 156.01 ) in 500 ml A. dest and adjust pH with 10 M NaOH (Sigma 72068, FW = 40.00) to 7.1.