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Abdel-Rahman 2016 Oxid Med Cell Longev
Additional label 2016-11  + , Amplex Ultra Red  +
Coupling states LEAK  + , OXPHOS  +
Has abstract Disruption of cellular redox homeostasis i
Disruption of cellular redox homeostasis is implicated in a wide variety of pathologic conditions and aging. A fundamental factor that dictates such balance is the ratio between mitochondria-mediated complete oxygen reduction into water and incomplete reduction into superoxide radical by mitochondria and NADPH oxidase (NOX) enzymatic activity. Here we determined mitochondrial as well as NOX-dependent rates of oxygen consumption in parallel with H<sub>2</sub>O<sub>2</sub> generation in freshly isolated synaptosomes using high-resolution respirometry combined with fluorescence or electrochemical sensory. Our results indicate that, although synaptic mitochondria exhibit substantially higher respiratory activities (8-82 folds greater than NOX oxygen consumption depending on mitochondrial respiratory state), NADPH-dependent oxygen consumption is associated with greater H<sub>2</sub>O<sub>2</sub> production (6-7 folds higher NOX-H<sub>2</sub>O<sub>2</sub>). We also show that, in terms of the consumed oxygen, while synaptic mitochondria ‘leaked’ 0.71% ± 0.12 H<sub>2</sub>O<sub>2</sub> during NAD<sup>+</sup>-linked resting, 0.21% ± 0.04 during NAD<sup>+</sup>-linked active, and 0.07% ± 0.02 during FAD<sup>+</sup>-linked active respirations, NOX converted 38% ± 13 of O<sub>2</sub> into H<sub>2</sub>O<sub>2</sub>. Our results indicate that NOX rather than mitochondria is the major source of synaptic H<sub>2</sub>O<sub>2</sub>. The present approach may assist in the identification of redox-modulating synaptic factors that underlie a variety of physiological and pathological processes in neurons.
cal and pathological processes in neurons.  +
Has info [ Open Access]  +
Has publicationkeywords ROS  + , Mitochondria  + , NADPH Oxidase  + , Synaptosomes  + , High-resolution respirometry  +
Has title Abdel-Rahman EA, Mokhtar A, Aaliya A, Radwan Y, Yasseen B, Al-Okda A, Atwa A, Elhanafy E, Habashy M, Ali SS (2016) Resolving contributions of oxygen-consuming and ROS-generating enzymes at the synapse. Oxid Med Cell Longev p19.  +
Instrument and method Oxygraph-2k  + , O2k-Fluorometer  +
Mammal and model Mouse  +
MiP area Respiration  +
Pathways N  + , NS  + , ROX  +
Preparation Permeabilized cells  +
Stress Oxidative stress;RONS  +
Tissue and cell Nervous system  +
Was published by MiPNetLab EG Cairo Ali SS +
Was published in journal Oxid Med Cell Longev +
Was published in year 2016  +
Was written by Abdel-Rahman EA + , Mahmoud AM + , Aaliya A + , Radwan Y + , Yasseen B + , Al-Okda A + , Atwa A + , Elhanafy E + , Habashy M + , Ali SS +
Categories Publications
Modification date
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08:11:42, 9 April 2018  +
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