Search by property

This page provides a simple browsing interface for finding entities described by a property and a named value. Other available search interfaces include the page property search, and the ask query builder.

Search by property

A list of all pages that have property "Has abstract" with value "Nitric oxide (NO) inhibits mitochondrial respiration by decreasing the apparent affinity of cytochrome c oxidase (CIV) for oxygen. Using iNOS-transfected HEK 293 cells to achieve regulated intracellular NO production, we determined NO and O2 concentrations and mitochondrial O2 consumption by high-resolution respirometry over a range of O2 concentrations down to nanomolar. Inhibition of respiration by NO was reversible, and complete NO removal recovered cell respiration above its routine reference values. Respiration was observed even at high NO concentrations, and the dependence of IC50 on [O2] exhibits a characteristic but puzzling parabolic shape; both these features imply that CIV is protected from complete inactivation by NO and are likely to be physiologically relevant. We present a kinetic model of CIV inhibition by NO that efficiently predicts experimentally determined respiration at physiological O2 and NO concentrations and under hypoxia, and accurately predicts the respiratory responses under hyperoxia. The model invokes competitive and uncompetitive inhibition by binding of NO to the reduced and oxidized forms of CIV, respectively, and suggests that dissociation of NO from reduced CIV may involve its O2 dependent oxidation. It also explains the non-linear dependence of IC50 on O2 concentration, and the hyperbolic increase of ''c''50 as a function of NO concentration.". Since there have been only a few results, also nearby values are displayed.

Showing below up to 2 results starting with #1.

View (previous 50 | next 50) (20 | 50 | 100 | 250 | 500)

Property: Value:

List of results

Aguirre 2010 Biochim Biophys Acta + (Nitric oxide (NO) inhibits mitochondrial r … Nitric oxide (NO) inhibits mitochondrial respiration by decreasing the apparent affinity of cytochrome c oxidase (CIV) for oxygen. Using iNOS-transfected HEK 293 cells to achieve regulated intracellular NO production, we determined NO and O2 concentrations and mitochondrial O2 consumption by high-resolution respirometry over a range of O2 concentrations down to nanomolar. Inhibition of respiration by NO was reversible, and complete NO removal recovered cell respiration above its routine reference values. Respiration was observed even at high NO concentrations, and the dependence of IC50 on [O2] exhibits a characteristic but puzzling parabolic shape; both these features imply that CIV is protected from complete inactivation by NO and are likely to be physiologically relevant. We present a kinetic model of CIV inhibition by NO that efficiently predicts experimentally determined respiration at physiological O2 and NO concentrations and under hypoxia, and accurately predicts the respiratory responses under hyperoxia. The model invokes competitive and uncompetitive inhibition by binding of NO to the reduced and oxidized forms of CIV, respectively, and suggests that dissociation of NO from reduced CIV may involve its O2 dependent oxidation. It also explains the non-linear dependence of IC50 on O2 concentration, and the hyperbolic increase of ''c''50 as a function of NO concentration.sub>50 on O2 concentration, and the hyperbolic increase of ''c''50 as a function of NO concentration.)

Aguirre 2010 Biochim Biophys Acta +