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Property "Has-20abstract" (as page type) with input value "-5B-5BImage:MITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http:-2F-2Fwww.mitoglobal.org-2Findex.php-2FMITOEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0ALETM1-20is-20an-20inner-20membrane-20protein-20of-20the-20mitochondria-20which-20its-20gene-20found-20to-20be-20deleted-20in-20Wolf-2DHirschhorn-20syndrome-20patients.-20It-20has-20been-20suggested-20that-20cellular-20damage-20caused-20by-20deletion-20of-20LETM1-20may-20be-20related-20to-20oxidative-20stress.-20It-20is-20well-20known-20that-20melatonin-20provides-20antioxidant-20enzyme-20activation-20to-20protect-20the-20cell-20and-20mitochondrion-20from-20oxidative-20stress-2Dinduced-20damage.-20In-20our-20study,-20in-20order-20to-20investigate-20the-20effects-20of-20LETM1-20loss-20on-20oxidative-20stress-20and-20melatonin-20on-20mitochondrial-20function-20in-20immortalized-20mouse-20embryonic-20fibroblast-20cells,-20to-20suppress-20the-20LETM1-20gene-20siRNA-20transfection,-20SDS-2DPAGE-20and-20BN-2DPAGE-20to-20decompose-20OXPHOS-20complexes-20and-20immunoblotting-20methods-20were-20used,-20to-20determine-20oxidative-20stress-20in-20cells,-20fluorescence-20microscopy,-20-20aconitase-20enzyme-20activity-20and-20protein-20oxidation-20determined,-20and-20oxygen-20consumption-20was-20measured-20in-20the-20presence-20of-20appropriate-20substrates-20and-20inhibitors-20in-20the-20cells.-20The-20expression-20of-20LETM1-20was-20suppressed-20by-20approximately-2050-25-20with-20siRNA-20transfection-20in-20MEFs-20and-20the-20application-20of-20melatonin-20to-20these-20cells-20did-20not-20alter-20the-20level-20of-20LETM1.-20It-20has-20been-20determined-20that-20MnSOD-20expression-20and-20aconitase-20activity-20are-20decreased-20and-20total-20protein-20oxidation-20is-20increased-20in-20transfected-20cells.-20After-20administration-20of-20melatonin,-20MnSOD-20expression,-20aconitase-20activity-20and-20protein-20oxidation-20were-20normalized.-20It-20was-20determined-20that-20LETM1-20suppression-20did-20not-20alter-20the-20expression-20and-20formation-20of-20OXPHOS-20complexes-20as-20determined-20by-20BN-2DPAGE,-20but-20the-20oxygen-20consumption-20rates-20decreased-20significantly.-20The-20change-20in-20oxygen-20consumption-20rate-20is-20not-20related-20to-20complex-20I-20but-20carried-20out-20on-20complex-20II-20shown-20for-20the-20first-20time-20in-20the-20literature-20and-20on-20complex-20IV-20in-20accordance-20with-20the-20literature.-20It-20was-20also-20determined-20that-20the-20reduced-20oxygen-20consumption-20rate-20was-20normalized-20by-20melatonin-20administration.-20These-20results-20provide-20contribution-20to-20the-20literature-20to-20clarify-20the-20effects-20of-20LETM1-20loss-20in-20relation-20to-20oxidative-20stress-20and-20melatonin-20on-20mitochondrial-20function." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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