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Property "Has-20abstract" (as page type) with input value "Although-20severe-20water-20loss-20is-20often-20detrimental-20to-20life,-20some-20exceptional-20animals-20have-20developed-20mechanisms-20to-20survive-20water-20loss-20to-200.02–0.05-20g-20H2O-2Fg-20dry-20mass-20(anhydrobiosis).-20How-20the-20structural-20and-20functional-20integrity-20of-20the-20mitochondrion-20is-20maintained-20during-20desiccation-20and-20rehydration-20is-20currently-20not-20understood.-20The-20brine-20shrimp-20Artemia-20franciscana-20serves-20as-20an-20important-20model-20organism-20for-20anhydrobiosis.-20Desiccation-20tolerance-20in-20this-20animal-20is-20correlated-20with-20the-20accumulation-20of-20large-20amounts-20of-20highly-20hydrophilic-20macromolecules-20termed-20Late-20Embryogenesis-20Abundant-20(LEA)-20proteins.-20Most-20LEA-20proteins-20are-20intrinsically-20disordered-20in-20solution-20and-20thought-20to-20stabilize-20other-20proteins-20and-20membranes-20during-20desiccation-20-5B1-5D.-20Two-20mitochondrial-20targeted-20LEA-20proteins,-20AfrLEA3m-20and-20AfLEA1.3,-20have-20been-20described-20in-20A.-20franciscana-20and-20may-20help-20to-20maintain-20the-20integrity-20and-20functionality-20of-20the-20organelle-20when-20water-20is-20scarce-20-5B2,-203-5D.-20-0A-0AIn-20order-20to-20evaluate-20the-20impact-20of-20AfLEA1.3-20on-20water-2Dstress-20tolerance-20of-20mitochondria-20the-20protein-20was-20transgenically-20expressed-20in-20Kc167-20cells-20from-20the-20desiccation-20intolerant-20fruit-20fly-20Drosophila-20melanogaster-20-5B4-5D.-20A-20protein-20construct-20composed-20of-20AfLEA1.3-20and-20green-20fluorescent-20protein-20was-20found-20to-20accumulate-20within-20mitochondria.-20Expression-20of-20AfLEA1.3-20in-20non-2Dpermeabilized-20cells-20reduced-20mitochondrial-20proton-20leak-20by-2020-2D30-25-20in-20presence-20and-20absence-20of-20hyperosmotic-20stress.-20Oxygen-20consumption-20of-20permeabilized-20cells-20in-20presence-20of-20mixed-20substrates-20and-20ADP-20was-2018-25-20less-20inhibited-20by-20increasing-20concentrations-20of-20NaCl-20in-20cells-20expressing-20AfLEA1.3-20compared-20to-20control-20cells.-20Mitochondria-20isolated-20from-20wild-2Dtype-20and-20AfLEA1.3-20expressing-20cells-20showed-20a-20high-20respiratory-20control-20ratio-20(RCR)-20of-2011.3-20±-201.4.-20Oxygen-20consumption-20in-20presence-20of-20ADP-20and-20substrates-20that-20supply-20NADH-20to-20Complex-20I-20were-20significantly-20reduced-20after-20freezing-20and-20thawing-20and-20this-20reduction-20was-20significantly-20greater-20in-20mitochondria-20from-20control-20cells-20(RCR-20=-207.3-20±-201.0)-20than-20in-20cells-20expressing-20AfLEA1.3-20(RCR-20=-2010.1-20±-200.7,-20-27-27P-27-27-3C-200.05).-20-0A-0AExpression-20of-20AfLEA1.3-20improved-20functions-20of-20mitochondria-20from-20D.-20melanogaster-20in-20several-20models-20of-20water-20stress.-20Protection-20was-20observed-20at-20hydration-20states-20in-20which-20AfLEA1.3-20most-20likely-20lacks-20extensive-20secondary-20structure-20and-20the-20protein-20was-20most-20likely-20intrinsically-20disordered.-20AfLEA1.3-20thus-20can-20operate-20outside-20the-20classic-20structure-20function-20paradigm.-20However,-20the-20observed-20effects-20afforded-20by-20the-20hydrated-20AfLEA1.3-20protein-20do-20not-20preclude-20even-20greater-20stabilization-20of-20biological-20structure-20and-20function-20in-20the-20dried-20state." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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