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Property "Has-20abstract" (as page type) with input value "Although-20the-20decrease-20of-20pyruvate-20secretion-20by-20brewer’s-20yeasts-20during-20fermentation-20has-20long-20been-20desired-20in-20the-20alcohol-20beverage-20industry,-20rather-20little-20is-20known-20about-20the-20regulation-20of-20pyruvate-20-20accumulation.-20In-20this-20study,-20we-20have-20characterized-20a-20previously-20developed-20a-20pyruvate-20undersecreting-20sake-20yeast-20obtained-20by-20isolating-20a-20strain-20(TCR7)-20tolerant-20to-20ethyl-20α-2Dtranscyanocinnamate,-20an-20inhibitor-20of-20pyruvate-20transport-20into-20mitochondria.-20To-20obtain-20insights-20into-20pyruvate-20metabolism,-20we-20investigated-20the-20mitochondrial-20activity-20of-20TCR7-20by-20oxigraphy-20and-2013C-2Dmetabolic-20flux-20analysis-20during-20aerobic-20growth-20.-20While-20mitochondrial-20pyruvate-20oxidation-20was-20higher,-20glycerol-20production-20was-20decreased-20in-20TCR7-20compared-20to-20the-20reference.-20These-20results-20indicate-20that-20mitochondrial-20activity-20is-20elevated-20in-20the-20TCR7-20strain-20with-20the-20consequence-20of-20decreased-20pyruvate-20-20accumulation.-20Surprisingly-20mitochondrial-20activity-20is-20much-20higher-20in-20the-20sake-20yeast-20compared-20to-20CEN.PK-20113-2D7D,-20the-20reference-20strain-20in-20metabolic-20engineering.-20When-20shifted-20from-20aerobic-20to-20anaerobic-20conditions,-20sake-20yeast-20retains-20a-20branched-20mitochondrial-20structure-20for-20a-20longer-20time-20than-20laboratory-20strains.-20The-20regulation-20of-20mitochondrial-20activity-20can-20become-20a-20completely-20novel-20approach-20to-20manipulate-20metabolic-20profile-20during-20fermentation-20of-20brewer’s-20yeasts.-0A-0A-5B-5BFile:Abstract-20Agrimi-20G-20Graphical.jpg-7Ccenter-7C450px-5D-5D" contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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