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Property "Has-20abstract" (as page type) with input value "We-20use-20the-20measurement-20of-20integrated-20mitochondrial-20function-20or-20oxidative-20phosphorylation-20(OXPHOS)-20to-20evaluate-20mitochondrial-20dysfunction-20in-20energy-20production-20as-20a-20diagnostic-20tool-20in-20skeletal-20muscle.-20-20During-20an-20one-20year-20period-20we-20used-20both-20isolated-20skeletal-20muscle-20mitochondria-20and-20permeabilized-20fibers-20from-20the-20same-20biopsy-20to-20evaluate-20OXPHOS-20capacity.-20-20-0A-0AThe-20goal-20of-20our-20study-20was-20to-20perform-20a-20quantitative-20and-20qualitative-20comparison-20of-20those-20two-20methods-20in-20muscle-20biopsy-20samples-20(-27-27vastus-20lateralis-27-27).-20-20The-20muscle-20sample-20from-2040-20patients-20was-20used-20for-20(1)-20isolation-20of-20mitochondria,-20and-20(2)-20muscle-20fibers-20permeabilization.-20Oxygen-20consumption-20was-20performed-20with-20the-20isolated-20mitochondria-20using-20a-20conventional-20oxygraph-20and-20standard-20protocols-20(performed-20by-20Mariana-20Rosca,-20M.D.-20-5B1-5D),-20while-20for-20the-20permeabilized-20fibers,-20high-2Dresolution-20respirometry-20combined-20with-20a-20multiple-20substrate-2Duncoupler-2Dinhibitor-2Dtitration-20(SUIT)-20protocol-20was-20used-20(-5B-5BVazquez-20E-7CEdwin-20Vazquez-5D-5D-20-5B2-5D).-20Citrate-20synthase-20activity-20(Hiral-20Patel),-20a-20marker-20of-20mitochondrial-20content,-20was-20compared-20in-20homogenate-20of-20muscle-20and-20permeabilized-20fibers-20removed-20from-20the-20chamber-20after-20the-20estimation-20of-20OXPHOS.-20In-20a-20group-20identified-20as-20“pathological-20controls”,-20a-20quantitative-20comparison-20of-20the-20OXPHOS-20capacities-20obtained-20was-20performed-20between-20the-20two-20methods-20(studies-20done-20by-20-5B-5BLemieux-20H-7CHelene-20Lemieux,-20Ph.D.-5D-5D).-20Oxygen-20flux-20in-20isolated-20mitochondria-20was-20expressed-20per-20g-20wet-20weight-20of-20muscle-20using-20the-20mitochondrial-20protein-20yield-20from-20the-20muscle,-20or-20per-20unit-20of-20citrate-20synthase-20activity.-20-20Furthermore,-20a-20factor-20was-20applied-20for-20the-20conversion-20of-20flux-20in-20isolated-20mitochondria-20measured-20at-2030-20°C-20to-20the-20corresponding-20flux-20in-20the-20permeabilized-20fibers-20measured-20at-2037-20°C-20-5B2-5D.-20-20Independent-20of-20how-20the-20data-20are-20expressed,-20the-20estimation-20of-20mitochondrial-20respiration-20for-20substrates-20entering-20at-20Complexes-20I,-20II,-20or-20IV-20were-20similar-20if-20not-20identical-20in-20both-20methods.-20The-20careful-20and-20quantitative-20evaluation-20of-20methods-20available-20for-20measurement-20of-20mitochondrial-20function-20is-20important-20for-20combining-20such-20results-20in-20a-20comparative-20data-20base-20-5B2-5D." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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