Difference between revisions of "Stadlmann 1999 Transplant Proc"
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|journal=Transplant Proc | |journal=Transplant Proc | ||
|abstract=PRODUCTION of reactive oxygen species is an important mechanism of cold ischemia-reperfusion (CIR) injury.<sup>1,2</sup> Among different activated oxygen species, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays a significant role.<sup>3,4</sup> However, the precise mechanism of H<sub>2</sub>O<sub>2</sub>-mediated cellular injury and the specific cellular sites of H2O2 attack are not well defined. Recent studies suggest that mitochondria are one of the main targets of early reperfusion injury in endothelial cells.<sup>5</sup> Thus, the purpose of this study was to evaluate short-term effects of H<sub>2</sub>O<sub>2</sub> on the mitochondrial respiratory chain and mitochondrial membrane potential (MMP) in endothelial cells. | |abstract=PRODUCTION of reactive oxygen species is an important mechanism of cold ischemia-reperfusion (CIR) injury.<sup>1,2</sup> Among different activated oxygen species, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays a significant role.<sup>3,4</sup> However, the precise mechanism of H<sub>2</sub>O<sub>2</sub>-mediated cellular injury and the specific cellular sites of H2O2 attack are not well defined. Recent studies suggest that mitochondria are one of the main targets of early reperfusion injury in endothelial cells.<sup>5</sup> Thus, the purpose of this study was to evaluate short-term effects of H<sub>2</sub>O<sub>2</sub> on the mitochondrial respiratory chain and mitochondrial membrane potential (MMP) in endothelial cells. | ||
|mipnetlab=AT_Innsbruck_Gnaiger E | |mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom | ||
|discipline=Mitochondrial Physiology, Biomedicine | |discipline=Mitochondrial Physiology, Biomedicine | ||
}} | }} | ||
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|tissues=Endothelial; Epithelial; Mesothelial Cell | |tissues=Endothelial; Epithelial; Mesothelial Cell | ||
|preparations=Intact Cell; Cultured; Primary | |preparations=Intact Cell; Cultured; Primary | ||
| | |couplingstates=OXPHOS | ||
|discipline=Mitochondrial Physiology, Biomedicine | |discipline=Mitochondrial Physiology, Biomedicine | ||
}} | }} | ||
Revision as of 15:28, 6 December 2012
| Stadlmann S, Amberger A, Kuznetsov AV, Rieger G, Hengster P, Margreiter R, Gnaiger E (1999) Does H2O2-mediated oxidative stress reproduce mitochondrial cold preservation/reoxygenation injury in endothelial cells? Transplant Proc 31: 993. |
Stadlmann S, Amberger A, Kuznetsov AV, Rieger G, Hengster P, Margreiter R, Gnaiger E (1999) Transplant Proc
Abstract: PRODUCTION of reactive oxygen species is an important mechanism of cold ischemia-reperfusion (CIR) injury.1,2 Among different activated oxygen species, hydrogen peroxide (H2O2) plays a significant role.3,4 However, the precise mechanism of H2O2-mediated cellular injury and the specific cellular sites of H2O2 attack are not well defined. Recent studies suggest that mitochondria are one of the main targets of early reperfusion injury in endothelial cells.5 Thus, the purpose of this study was to evaluate short-term effects of H2O2 on the mitochondrial respiratory chain and mitochondrial membrane potential (MMP) in endothelial cells.
• O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT Innsbruck MitoCom
Labels:
Stress:Ischemia-Reperfusion; Preservation"Ischemia-Reperfusion; Preservation" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Human Tissue;cell: Endothelial; Epithelial; Mesothelial Cell"Endothelial; Epithelial; Mesothelial Cell" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k
