Difference between revisions of "TMPD"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Tm | |abbr=Tm | ||
|description=N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>.2HCl; applied as an artificial substrate for reducing cytochrome c in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration. | |description=N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>.2HCl; applied as an artificial substrate for reducing cytochrome ''c'' in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration. | ||
|info=MiPNet06.06, MiPNet09.12 | |info=MiPNet06.06, MiPNet09.12 | ||
|type=Substrate ETS | |type=Substrate ETS | ||
}} | }} | ||
| Line 8: | Line 8: | ||
|instruments=Chemicals; Media | |instruments=Chemicals; Media | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|preparations=Oxidase; Biochemical Oxidation, Enzyme | |||
|kinetics=Reduced Substrate; Cytochrome c | |kinetics=Reduced Substrate; Cytochrome c | ||
|enzymes=Complex IV; Cytochrome c Oxidase | |||
}} | }} | ||
Revision as of 23:17, 18 September 2010
- high-resolution terminology - matching measurements at high-resolution
TMPD
Description
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride, C10H16N2.2HCl; applied as an artificial substrate for reducing cytochrome c in the respirometric assay for cytochrome c oxidase activity; is maintained in a reduced state by ascorbate; undergoes autooxidation as a function of oxygen pressure, ascrobate and cytochrome c concentration.
Abbreviation: Tm
Reference: MiPNet06.06, MiPNet09.12
Labels:
Preparation: Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Enzyme
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
