Difference between revisions of "Talk:Amplex UltraRed"
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=== Comparison MiR05Cr to MiRK03Cr === | === Comparison MiR05Cr to MiRK03Cr === |
Revision as of 19:17, 6 February 2016
Comparison MiR05Cr to MiRK03Cr
Instrumental settings
- Amp. Gain 1000
- Light intesity variable: 1 mA, 2 mA and 5 mA
- Configuration O2k-Fluo sensor always the same: O2k P1: Sensors B-0111/B-0082; O2k P2: Sensors B-0119/B-0117; O2k P3: Sensors A-0004/B-0112; O2k P4: Sensors A-0087/A-0088.
Media
- MiR05Cr
- MiRK03Cr: KCl 130 mM, HEPES free acid 20 mM, KH2PO4 10 mM, MgCl2 3 mM, EGTA 0.5 mM, BSA 0.1%, pH=7, add 120 mg Creatine to 40 ml buffer. After thawing MiRK03 a preciptate was observed, which was dissolved after 20 min shaking.
Titrations
- 10 µl Amplex UltraRed (stock: 1 mM -> 5 µM final in chamber)
- 4 µl HRP (stock: 500 U/ml -> 1 U/ml final in chamber)
- 2*10 µl of 56,32 µM H2O2 solution
Results/Figures
Drift vs Sensitivity:
Fig.6
Fig.7
--> Drift and sensitivity at least as good as MitoOx2 (=MiRK02)
Drift vs light intensity
Fig.14 and Fig. 15
--> MirK03, MiR05: drift not dependent on light intensity ( 1 to 5 mA)
compare to "MitoOx2":
Evaluation of respirometry in MiR05Cr versus MiRK03Cr (N=2):
Titrations
- 10 µl Amplex UltraRed (stock: 1 mM -> 5 µM final in chamber)
- 4 µl HRP (stock: 500 U/ml -> 1 U/ml final in chamber)
- 2*5 µl of 28.16 µM H2O2 solution : 0 µM, 0.07040 µM, 0.1406 µM
Fig.16 and 17
Fontanam 16:22, 6 August 2013 (CEST)
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