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| The use of AmR in HRR to simultaneously determine respiration and H2O2 fluxes has been intensively tested in the OROBOROS lab and there are several points to be considered in its application:
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| ::::* AmR, similar to other chemical probes, exerts a certain degree of toxicity and should thus be used at the lowest concentration still allowing a proper detection of H2O2 production rates.
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| ::::* Even in the absence of any sample, there is a spontaneous increase of the resorufin fluorescence signal over time detectable, the extent of which depends on components of the respiration medium used. Further, the assay sensitivity – the change in fluorescence per unit of H2O2 produced – is dependent on the medium used and tends to decline over time. Both the background change in fluorescence and the change in assay sensitivity over time need to be corrected for when analyzing results of H2O2 production experiments.
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| ::::* Media that may minimize unwanted effects on fluorescence may not necessarily be optimal to support respiration and thus need to be carefully selected. At present, MiR05Cr and the new medium MiR07Cr (still under evaluation) appear to be the most suitable media for simultaneous respirometry and H2O2 flux detection.
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| [[Image:BB-Bioblast.jpg|left|30px|link=Bioblast:About|Bioblast wiki]] | | [[Image:BB-Bioblast.jpg|left|30px|link=Bioblast:About|Bioblast wiki]] |
| == Popular Bioblast page == | | == Popular Bioblast page == |
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