Difference between revisions of "Talk:Amplex UltraRed"
From Bioblast
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{| style="border-spacing:0;" | {| border="1" style="border-spacing:0;" style="text-align: center;" | ||
|| Citation | || Citation | ||
| colspan="2" | Amplex | | colspan="2" | Amplex | ||
| colspan="3" | HRP | | colspan="3" | HRP | ||
|| pH | || pH | ||
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|- | |- | ||
|| | || | ||
|| | || stock | ||
|| | || final | ||
|| | || unit definition | ||
|| | || stock | ||
|| | || final | ||
|| | || | ||
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Revision as of 15:51, 19 August 2013
Different Brands
| Trade Mark | Manufacturer/ Distributor | price [€/mg]
|
product id
|
| Amplex Red | 37.4
|
A12222
| |
| Amplex Ultra Red | 48.8
|
A36006
| |
| Ampliflu Red | 18.5
|
90101
| |
| Quanta Red | 15150
|
Conditions
| Citation | Amplex | HRP | pH | Limit of detection | |||
| stock | final | unit definition | stock | final | |||
mM
|
µM
|
U/ml
|
U/ml
|
||||
| BIOTEK | 10
|
50
|
pyrogallol
|
10
|
0.1
|
7.4
|
4 nM (absorption 300 nm)
|
| INVITROGEN | 10
|
50
|
10
|
0.1
|
6 to 7.5
|
<80 nM
| |
| Towne 2004 | 160
|
0.41
|
7.5 to 8.5
|
100 nM
| |||
| Zhou1997 | 3 ?
|
0.3 to 1
|
50 nM (10 nm optimal cond)
| ||||
| Mohanty1997 | 100
|
10 to 100
|
1
|
18 nM
| |||
| Komary2010 | 1
|
2.5
|
|||||
At Oroboros Instruments we use the publication from Prof. Tretters OROBOROS MiPNet Reference Laboratory as a starting point for our development.
