Difference between revisions of "Talk:Amplex UltraRed"
From Bioblast
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At Oroboros Instruments we | At Oroboros Instruments we used two publications from [[HU Budapest Tretter L|Prof. Tretters OROBOROS MiPNet Reference Laboratory]] as a starting point for our development: [[Tretter 2012 Free Radic Biol Med]] and [[Komary 2010 Biochim Biophys Acta]]. | ||
== Media == | == Media == | ||
Revision as of 09:38, 17 September 2013
Different Brands
| Trade Mark | Manufacturer/ Distributor | price [€/mg]
|
product id
|
| Amplex Red | 37.4
|
A12222
| |
| Amplex Ultra Red | 48.8
|
A36006
| |
| Ampliflu Red | 18.5
|
90101
| |
| Quanta Red | 15150
|
Conditions
| Citation | Amplex | HRP | pH | Limit of detection | |||
| stock | final | unit definition | stock | final | |||
mM
|
µM
|
U/ml
|
U/ml
|
||||
| BIOTEK | 10
|
50
|
pyrogallol
|
10
|
0.1
|
7.4
|
4 nM (absorption 300 nm)
|
| INVITROGEN | 10
|
50
|
10
|
0.1
|
6 to 7.5
|
<80 nM
| |
| Towne 2004 | 160
|
0.41
|
7.5 to 8.5
|
100 nM
| |||
| Zhou1997 | 3 ?
|
0.3 to 1
|
50 nM (10 nm optimal cond)
| ||||
| Mohanty1997 | 100
|
10 to 100
|
1
|
18 nM
| |||
| Komary2010 | 1
|
2.5
|
|||||
At Oroboros Instruments we used two publications from Prof. Tretters OROBOROS MiPNet Reference Laboratory as a starting point for our development: Tretter 2012 Free Radic Biol Med and Komary 2010 Biochim Biophys Acta.
