Talk:Glucose

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MiPNet discussion forum: problem with Omy (2013-10-22)

Dominik Pesta

Has anyone ever looked at glucose oxidation in intact skeletal muscle fibres (of mouse muscle)? I have tried with a Krebs Henseleit Buffer (no substrates) and Malate and/or Pyruvate followed by glucose (basal) and insulin titration (insulin stimulated) but do not get any stimulation of respiration. Although malate will not permeate the cell, I would at least expect some stimulation with glucose. Any thoughts on that?

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