Talk:Malate: Difference between revisions
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In the choice of adequate substrates for the evaluation of mitochondrial energetics it is not only the nature but also the quantity that is of crucial importance. Accordingly, we recently tested the concentration of Malate when added together with Pyruvate and/or Glutamate and observed that while Malate stimulates respiration at 0.5 mM concentration, it becomes inhibitory at the generally used concentration of 2 mM. This has been documented for fish liver and heart homogenates (post [http://www.oroboros.at/?IOC80_schroecken#c3877| IOC80] training course), mouse heart, liver and brain homogenate preparations, dog skeletal muscle fibers ([http://www.oroboros.at/index.php?id=ioc84_alaska| IOC84]), beef heart mitochondria, and brine shrimp (''Artemia franciscana'') mitochondria. | In the choice of adequate substrates for the evaluation of mitochondrial energetics it is not only the nature but also the quantity that is of crucial importance. Accordingly, we recently tested the concentration of Malate when added together with Pyruvate and/or Glutamate and observed that while Malate stimulates respiration at 0.5 mM concentration, it becomes inhibitory at the generally used concentration of 2 mM. This has been documented for fish liver and heart homogenates (post [http://www.oroboros.at/?IOC80_schroecken#c3877| IOC80] training course), mouse heart, liver and brain homogenate preparations, dog skeletal muscle fibers ([http://www.oroboros.at/index.php?id=ioc84_alaska| IOC84]), beef heart mitochondria, and brine shrimp (''Artemia franciscana'') mitochondria. | ||
Some representative examples will be displayed here soon. | |||
Revision as of 12:45, 7 February 2014
In the choice of adequate substrates for the evaluation of mitochondrial energetics it is not only the nature but also the quantity that is of crucial importance. Accordingly, we recently tested the concentration of Malate when added together with Pyruvate and/or Glutamate and observed that while Malate stimulates respiration at 0.5 mM concentration, it becomes inhibitory at the generally used concentration of 2 mM. This has been documented for fish liver and heart homogenates (post IOC80 training course), mouse heart, liver and brain homogenate preparations, dog skeletal muscle fibers (IOC84), beef heart mitochondria, and brine shrimp (Artemia franciscana) mitochondria.
Some representative examples will be displayed here soon.
