Talk:Malate: Difference between revisions

Revision as of 17:47, 26 February 2014

Optimum malate concentration in SUIT protocols

A MiPNet publication in preparation by Krumschnabel G, Sumbalova Z, Schöpf B, Burtscher J, Eigentler A, Fontana-Ayoub M, Heidler J, Laner V, Kotb IA, Salin K, Hand SC, Ali SS, Gnaiger E et al.

The type and concentration of respiratory substrates is important for the design of substrate-uncoupler-inhibitor titration (SUIT) protocols.

0.5 mM malate saturates CI linked respiration in combination with pyruvate and/or glutamate, whereas 2 mM malate inhibits CII linked (succinate+rotenone) and CI+II linked respiration, as shown in mouse heart (collaboration with the group of Sameh Ali), mouse brain homogenate, beef heart mitochondria, human permeabilized skeletal muscle fibres, dog permeabilized skeletal muscle fibers (IOC84), brine shrimp embryo (Artemia franciscana) mitochondria (collaboration with Steve Hand), fish liver and heart homogenates (post IOC80 training course).

Some representative examples are presented here.

Arctic charr liver homogenate

Fig. 1. Arctic charr liver homogenate (3.99 mg/ml, 15 °C): GM0.5+D5+c+P+S+reox+U1+U2+U3+U3.5+Rot+M1+M2. Liver homogenate respiring at CII linked ETS were given 1 mM and 2 mM malate (M1 and M2), which inhibited respiration by 1.1% and 13%, respectively. Experiments post IOC80; data evaluation by Karine Saline.

Arctic charr heart homogenate

Fig. 2. Arctic charr heart homogenate (1.05 mg/ml, 15°C): G+M0.5+D5+c+P+S+reox+U1+U2+U3+U3.5+Rot(not shown)]+M1+M2+Car+Mna+Ama. Heart homogenate respiring at CII linked ETS was given 1 mM and 2 mM malate (M1 and M2), which inhibited respiration by 7% and 15%, respectively. Adding carrier (Car) inhibited only 0.9%. Experiments post IOC80; data evaluation by Karine Saline.

Mouse brain homogenate

Fig. 3. Mouse brain homogenate (0.9 mg/ml): Rot+S+D2.5+Car1+M1+M2+Car2. Addition of the carrier water (Car1, Car2) had no significant impact on CII linked OXPHOS (inhibition by 2.2% and -0.2%), whereas the addition of 1 mM and 2 mM Malate (M1 and M2) inhibited respiration by 21% and 11%, respectively. Experiment in the OROBOROS MitoLab and data evaluation by Kotb IA.

Beef heart mitochondria

Fig. 4. Beef heart mitochondria: Rot+S+D2.5+M2. Beef heat mitochondria respiring at CII linked OXPHOS were given 2 mM malate (M2), which inhibited respiration by 31%. Experiment in the OROBOROS MitoLab by Heidler J et al.

Brine shrimp embryo mitochondria

Fig. 5. Brine shrimp embryo mitochondria: Rot+S+D2.5+cyt c+ U+M0.5 (not shown)+M2+Ama. Brine shrimp embryo mitochondria respiring at CII linked ETS with 0.5 mM Malate were given 2 mM malate (M2), which inhibited respiration by 12%. Experiment in the OROBOROS MitoLab by Steve Hand et al.

@@ Line 9: / Line 9: @@
-'''Arctic char liver homogenate:'''
+'''Arctic charr liver homogenate'''
 [[Image:Char liver homogenate Malate effect.png|600px]]
-Fig.1. Arctic Char liver homogenate (3.989 mg/ml, 15°C): GM0.5+D5+c+P+S+Reox+U1+U2+U3+U3.5+Rot+M1+M2. Liver homogenate respiring at CII-linked ETS were given 1 mM Malate (M1) and 2 mM Malate (M2), which inhibited respiration by 1.1% and 13%, respectively. (Data evaluation by [[Salin K|Karine Saline]]).
+Fig. 1. Arctic charr liver homogenate (3.99 mg/ml, 15 °C): GM0.5+D5+c+P+S+reox+U1+U2+U3+U3.5+Rot+M1+M2. Liver homogenate respiring at CII linked ETS were given 1 mM and 2 mM malate (M1 and M2), which inhibited respiration by 1.1% and 13%, respectively. Experiments post IOC80; data evaluation by [[Salin K|Karine Saline]].
-'''Arctic char heart homogenate:'''
+'''Arctic charr heart homogenate'''
 [[Image:Char heart homogenate Malate effect.png|600px]]
-Fig.2. Arctic Char heart homogenate (1.054 mg/ml, 15°C): [G+M0.5+D5+c+P+S+Reox+U1+U2+U3+U3.5+Rot(not shown)]+M1+M2+Car+Mna+Ama. Heart homogenate respiring at CII-linked ETS was given 1 mM Malate (M1) and 2 mM Malate (M2), which inhibited respiration by 7% and 15%, respectively. Adding Carrier inhibited only 0.9%. (Data evaluation by [[Salin K|Karine Saline]]).
+Fig. 2. Arctic charr heart homogenate (1.05 mg/ml, 15°C): G+M0.5+D5+c+P+S+reox+U1+U2+U3+U3.5+Rot(not shown)]+M1+M2+Car+Mna+Ama. Heart homogenate respiring at CII linked ETS was given 1 mM and 2 mM malate (M1 and M2), which inhibited respiration by 7% and 15%, respectively. Adding carrier (Car) inhibited only 0.9%. Experiments post IOC80; data evaluation by [[Salin K|Karine Saline]].
-'''Mouse brain homogenate:'''
+'''Mouse brain homogenate'''
 [[Image:Mouse brain homogenate Malate effect.png|600px]]
-Fig.3. Mouse brain homogenate (0.9 mg/ml): Rot+S+D2.5+Car1+M1+M2+Car2. Addition of the carrier water (Car1, Car2) had no significant impact on CII-linked OXPHOS (inhibition by 2.2% and -0.2%), whereas the addition of 1 mM Malate (M1) and 2 mM Malate (M2) inhibited respiration by 21% and 11%, respectively.(Experiment and data evaluation by [[Kotb IA]])
+Fig. 3. Mouse brain homogenate (0.9 mg/ml): Rot+S+D2.5+Car1+M1+M2+Car2. Addition of the carrier water (Car1, Car2) had no significant impact on CII linked OXPHOS (inhibition by 2.2% and -0.2%), whereas the addition of 1 mM and 2 mM Malate (M1 and M2) inhibited respiration by 21% and 11%, respectively. Experiment in the OROBOROS MitoLab and data evaluation by [[Kotb IA]].
-'''Beef heart mitochondria:'''
+'''Beef heart mitochondria'''
 [[Image:Beef heart Mitochondria Malate effect.png|600px]]
-Fig.4. Beef heart mitochondria: Rot+S+D2.5+M2. Beef heat mitochondria respiring at CII-linked OXPHOS were given 2 mM Malate (M2), which inhibited respiration by 31%.
+Fig. 4. Beef heart mitochondria: Rot+S+D2.5+M2. Beef heat mitochondria respiring at CII linked OXPHOS were given 2 mM malate (M2), which inhibited respiration by 31%. Experiment in the OROBOROS MitoLab by [[Heidler J]] et al.
-'''Brine shrimp embryo mitochondria:'''
+'''Brine shrimp embryo mitochondria'''
 [[Image:Brine shrimp embryo mitochondria Malate effect.png|600px]]
-Fig.5. Brine shrimp embryo mitochondria: [Rot+S+D2.5+cyt c+ U+M0.5 (not shown)]+M2+Ama. Brine shrimp embryo mitochondria respiring at CII-linked ETS with 0.5 mM Malate were given 2 mM Malate (M2), which inhibited respiration by 12%. (Experiment by [[US_LA_Baton_Rouge_Hand_SC|Steve Hand]]).
+Fig. 5. Brine shrimp embryo mitochondria: Rot+S+D2.5+cyt c+ U+M0.5 (not shown)+M2+Ama. Brine shrimp embryo mitochondria respiring at CII linked ETS with 0.5 mM Malate were given 2 mM malate (M2), which inhibited respiration by 12%. Experiment in the OROBOROS MitoLab by [[US_LA_Baton_Rouge_Hand_SC|Steve Hand]] et al.