Talk:MiPNet21.16 DatLab 7 Innovations

DatLab 7.3 Innovations

*With new DatLab Protocols as guide through your measurements and a quick reference on the significance of each protocol step

*Includes DatLab protocols for instrumental calibration and maintenance and a collection of more than 20 Substrate-Uncoupler-Inhibitor-Titration (SUIT) protocols.

*With easy access to a library of protocols for browsing and selection of the appropriate SUIT protocol and with example traces for each protocol and explanatory text.

*SUIT protocols include optimum tested, but adjustable oxygen limits, and allow modification and storage of injection volumes and concentrations

*With a link for easy updates of novel SUIT protocols.

DatLab 7.3 Innovation - Functions explained in details

[Oroboros O2k]\O2k channel labels: Default channel labels can now be changed, and new labels set by the user. E.g., rename the Amperometric channel, Amp, to 'H2O2' for H2O2 measurements by fluorometry; rename the potentiometric channel, pX, to TPP+ for mitochondrial membrane measurements with the O2k-pH ISE-Module. For changing the label, go to menu [Oroboros O2k]\O2k channel labels and set the new channel label as desired.

[Protocols]\SUIT: Browse DL-Protocols and templates: A comprehensive set of new SUIT protocols with example traces with instructions and brief explanatory texts and templates for data evaluation can browed from inside DatLab 7.3. Click on menu [Protocols]\SUIT: Browse DL-Protocols and templates to open a folder with all the SUIT protocols provided with the USB. DatLab protocols (DLP) for different mitochondrial preparations can be chosen to assess multiple sequences of respiratory coupling and substrate states. Protocol possess unique D## codes and comprise a fixed sequence of events and marks which cannot be changed by the user. However, the user may edit concentrations and titration volumes of injections and store the modified protocol as user-specific DL-Protocol [File]\Export\DL-Protocol User (*.DLPU)]. For user support, we also provide PowerPoint files with explanatory cartoons and excel templates for data evaluation. If users wish to alter the nature of the chemicals used or the sequence of injections, we ask them to contact support@oroboros.at.

Lower O2 limit [μM]: Lower O2 limits optimized by the Oroboros_MitoFit_Laboratory are part of the DL-protocols provided. O2 limits can be individually set for each chamber and modified by the users. When the pre-set value is reached an automatic WARNING is triggered to remind the user that re-oxygenation of the medium may be required. To modify the O2 limit, select [Protocos]\Run DL-Protocol\Set O2 limit, tick the box next to Lower O2 limit [µM], enter desired value and click on Apply to chamber A or alternative as needed.

[Protocols]\Instrumental: Browse DL-Protocols and templates: DL-Protocols for instrumental calibration and maintenance can be browed from inside DatLab 7.3, example traces with instructions and brief explanatory texts and templates for data evaluation can be opened. Click on menu [Protocols]\Instrumental: Browse DL-Protocols and templates to open a folder with all the DatLab protocols (DLP) for cleaning, calibration, and background determination provided with the USB. Select a sub-directory and open an example trace and/or template as desired.

[Protocols]\Install Oroboros protocol package: Updates and newly developed DL protocols can be simply downloaded by clicking on [Protocols]\Install Oroboros protocol package.

[Protocols]\Browse www.bioblast.at/index.php./Mito-Pedia SUIT: Link to the web with the library of protocols and updates if available