Difference between revisions of "Talk:MiR05"

Latest revision as of 15:12, 7 August 2019

Towards the MiR05-Kit

Powder mix to transport Medium easily: (information provided by Cathrine Le)

Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.

Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.

Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.

Popular Bioblast page

MiR05 has been accessed more than

10,000 times (2019-08-07)
5,000 times (2016-08-08)

@@ Line 1: / Line 1: @@
+== Towards the MiR05-Kit ==
-Powder mix to transport Medium easily:(information provided by Cathrine Le)
-Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.
-Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.
+:::: Powder mix to transport Medium easily: (information provided by Cathrine Le)
+:::: Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.
-Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--[[User:Fontana Mona|Fontana Mona]] 11:02, 18 June 2013 (CEST)
+:::: Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.
-'''Mitochondrial respiration medium:'''
-A mitochondrial respiration medium is designed to support optimum mitochondrial function in the actual respirometric measurement. From the many media used by various groups the composition of 3 examples is shown:
-[[File:Respirometric_media.png|left|400px]]
-References:
+:::: Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.
-Buffer Z: [http://bioblast.at/index.php/Perry_2011_Biochem_J  Perry 2011 Bichem J]
-MiRK03: [http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], modified after [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta]
+[[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]
+== Popular Bioblast page ==
-MiR05: [http://bioblast.at/index.php/MiPNet14.13_Medium-MiR06 MiPNet14.03], [http://bioblast.at/index.php/Gnaiger_2000_Life_in_the_Cold Gnaiger 2000 Life in the Cold]
+::: [[MiR05]] has been accessed more than
+::::*  10,000 times (2019-08-07)
+::::*  5,000 times (2016-08-08)