Difference between revisions of "Talk:MiR05"
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Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--[[User:Fontana Mona|Fontana Mona]] 11:02, 18 June 2013 (CEST) | Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--[[User:Fontana Mona|Fontana Mona]] 11:02, 18 June 2013 (CEST) | ||
'''Mitochondrial respiration medium:''' | |||
A mitochondrial respiration medium is designed to support optimum mitochondrial function in the actual respirometric measurement. From the many media used by various groups the composition of 3 examples is shown: | |||
add Table here | |||
References: | |||
Buffer Z: [http://bioblast.at/index.php/Perry_2011_Biochem_J Perry 2011 Bichem J] | |||
MiRK03: [http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], modified after [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta] | |||
MiR05: [http://bioblast.at/index.php/MiPNet14.13_Medium-MiR06 MiPNet14.03], [http://bioblast.at/index.php/Gnaiger_2000_Life_in_the_Cold Gnaiger 2000 Life in the Cold] | |||
Revision as of 14:04, 22 September 2014
Powder mix to transport Medium easily:(information provided by Cathrine Le)
Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.
Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.
Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--Fontana Mona 11:02, 18 June 2013 (CEST)
Mitochondrial respiration medium:
A mitochondrial respiration medium is designed to support optimum mitochondrial function in the actual respirometric measurement. From the many media used by various groups the composition of 3 examples is shown:
add Table here
References:
Buffer Z: Perry 2011 Bichem J
MiRK03: Krumschnabel 2014 Methods Mol Biol, modified after Komary 2010 Biochim Biophys Acta
