Difference between revisions of "Talk:MiR05"
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Revision as of 14:53, 22 September 2014
Powder mix to transport Medium easily:(information provided by Cathrine Le)
Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.
Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.
Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--Fontana Mona 11:02, 18 June 2013 (CEST)
Mitochondrial respiration medium:
A mitochondrial respiration medium is designed to support optimum mitochondrial function in the actual respirometric measurement. From the many media used by various groups the composition of 3 examples is shown:
References:
Buffer Z: Perry 2011 Bichem J
MiRK03: Krumschnabel 2014 Methods Mol Biol, modified after Komary 2010 Biochim Biophys Acta
