Difference between revisions of "Talk:MiR05"

Revision as of 14:32, 4 December 2014

Powder mix to transport Medium easily:(information provided by Cathrine Le)

Regarding the "powder mix:" I brought up mixes of BIOPS and MiR06, both according to your recipes. I mixed the dry chemicals of the BIOPS in a 1L nalgene bottle and added the stock liquids of both CaK2EGTA and K2EGTA during pH'ing.

Additionally, I did the same for the MiR06. All dry chemicals were mixed in a 1L nalgene bottle with the exclusion of lactobionic acid which was stored separately/dry and made when we needed to make the MiR06.

Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--Fontana Mona 11:02, 18 June 2013 (CEST)

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 Both mixed powders for the MiR06 and BIOPS seem to do alright. I did try to keep them cold throughout transport, i.e. on ice.--[[User:Fontana Mona|Fontana Mona]] 11:02, 18 June 2013 (CEST)
-'''Mitochondrial respiration medium:'''
-A mitochondrial respiration medium is designed to support optimum mitochondrial function in the actual respirometric measurement. In some cases alterations of the formulation are incorporated to allow for the simultaneous measurement of additional parameters by e.g. fluorescence detection. From the many media used by various groups the composition of 3 examples is shown:
-[[File:Respirometric_media.png|400px]]
-References:
-Buffer Z: [http://bioblast.at/index.php/Perry_2011_Biochem_J  Perry 2011 Bichem J]
-MiRK03: [http://bioblast.at/index.php/Krumschnabel_2014_Methods_Mol_Biol Krumschnabel 2014 Methods Mol Biol], modified after [http://bioblast.at/index.php/Komary_2010_Biochim_Biophys_Acta Komary 2010 Biochim Biophys Acta]
-MiR05: [http://bioblast.at/index.php/MiPNet14.13_Medium-MiR06 MiPNet14.03], [http://bioblast.at/index.php/Gnaiger_2000_Life_in_the_Cold Gnaiger 2000 Life in the Cold]