Difference between revisions of "Talk:MitoPedia: Oroboros QM"

Latest revision as of 11:46, 16 December 2019

Work in progress

Experimental design

Experimental design: Insert a link to the project/manuscript page, where the rationale of the experiment (or experimental series) is summarized. Explain the burning question. Define the working hypothesis to predict the result.
Restructuring: It is not possible to book an O2k without proper documentation of the experimental design. If there is any doubt about the 'proper experimental design', a project meeting is to be organized in advance, to discuss the proposed and documented experimental details. Links to currently funded projects are generally helpful, and the rationale should be expanded on the relevant project/manuscript page.
A brief summary of the information obtained by a previous experiment is an important (or necessary) basis for the design of the next experiment. The summary has to be entered into the relevant project/manuscript page. An exception is only a time-series of experiments with a time-stamped, statistically planned and rigorously documented experimental protocol.
Please, enter here your further throughts, suggestions or questions, and confirm that you have been properly informed about our specific 'restructuring' which applies to both MitoFit labs (ORO and DSL).

** ~ Gnaigere (talk) 08:23, 22 May 2017 (CEST)

Keywords - definitions to be considered

Popular Bioblast page

MitoPedia: Oroboros QM has been accessed more than

5,000 times (2019-12-16)

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+__TOC__
   Work in progress
-== Keywords - definitions ==
+== Experimental design ==
-* [[MitoFit data repository]] - [[MitoFit data bank]]
+::::* '''Experimental design''': Insert a link to the project/manuscript page, where the rationale of the experiment (or experimental series) is summarized. Explain the '''burning question'''. Define the '''working hypothesis''' to '''predict''' the result.
+::::* '''Restructuring''': It is not possible to book an O2k without proper documentation of the experimental design. If there is any doubt about the 'proper experimental design', a project meeting is to be organized in advance, to discuss the proposed and documented experimental details. Links to currently funded projects are generally helpful, and the rationale should be expanded on the relevant project/manuscript page.
+::::* A brief '''summary''' of the information obtained by a previous experiment is an important (or necessary) basis for the design of the next experiment. The summary has to be entered into the relevant project/manuscript page. An exception is only a time-series of experiments with a time-stamped, statistically planned and rigorously documented experimental protocol.
+::::* Please, enter here your further throughts, suggestions or questions, and confirm that you have been properly informed about our specific 'restructuring' which applies to both MitoFit labs (ORO and DSL).
+:::::: ** ~ [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 08:23, 22 May 2017 (CEST)
-* [[Experiment]]
-:* [[Assay |Experimental assay]]
-:* [[Assay series |Experimental assay series]]
-:* [[Dataset]]
-* [[Sample]]
+== Keywords - definitions to be considered ==
-:* [[Sample type]]
-:* [[Sample group]]
-:* [[Sample size]]
-:* [[Population]]
-* [[MitoFit protocols]]
+# [[MitoFit data repository]] - [[MitoFit data bank]]
-:* [[Laboratory protocols]]
+# [[MitoFit team]]
-:* [[Harmonization protocols]] (Begley, Ioannidis 2015)
+## [[MitoFit moderator]]
-:* [[Solution protocols]]: media, substrates, uncouplers, inhibitors used in SUIT protocols, permeabilization agents, etc.
+## [[MitoFit accreditation panel]]
-:* [[Instrumental performance protocols]]
-:* [[Mitochondrial preparation protocols]] including biopsy sampling, tissue storage, cell preparation protocols, etc.
-:* [[Respirometric OXPHOS data acquisition and analysis protocols]]
-:* [[Normalization protocols]]
-* [[MitoFit proficiency test]]
+[[Image:BB-Bioblast.jpg|left|40px|link=http://www.bioblast.at/index.php/Bioblast:About|Bioblast wiki]]
+== Popular Bioblast page ==
-:* [[Standardization]]
+::: [[MitoPedia: Oroboros QM]] has been accessed more than
-:* [[Harmonization]]
+::::*  5,000 times (2019-12-16)
-:* [[Standard operating procedures]]
-:* [[Subprotocols]]
-* [[MitoFit team]]
-:* [[MitoFit moderator]]
-:* [[MitoFit accreditation panel]]
-* [[MitoFit registered project]]
-:* [[MitoFit pre-registered project]]
-:* [[Prespecified protocols]] (Begley, Ioannidis 2015)
-:* [[Time-stamped protocols]] (Begley, Ioannidis 2015)
-:* [[Publicly deposited protocols]] (Begley, Ioannidis 2015)
-== Work flow ==
-For registration of protocols, a project is announced with reference to:
-=== MitoPedia protocols ===
-* Sample preparation protocols
-* Solution protocols
-* Chemicals
-* Procedure protocols: (a) Platform-specific (ORO SOPs); (b) General (e.g. O2 calibratio principles).
-* SUIT protocols
-MitoPedia protocols are moderated by the [[MitoFit moderators]] (MitoFit team), either as protocols with direct reference to publications available to the scientific communicty, or protocols additionally described and made available in Bioblast with full information on authors (including contact details), author contribuitons, and editor (moderator) in charge.
-=== Pre-registration of a project ===
-Pre-registration of a project requires submission to a MitoFit moderator (editor) the protocol details with reference to MitoPedia protocols, or with submission of protocols for publication (Open Access) in MitoPedia. The MitoFit (Bioblast) editors will edit the submitted protocols (layout) and insert into Bioblast submitted pre-registrations and protocols. Guidelines will be defined.
-=== MitoFit accreditation ===
-The MitoFit moderators (editors) will set up a [[MitoFit accreditation panel]], into which the registrant will be included (perhaps not in the long run, to avoid conflict of interests) and/or for which the registrant can suggest delegates (compare peer review).
-Accredited MitoPedia protocols are (additionally) labelled as [[MitoFit protocols]].
-After accreditation of a pre-registered MitoFit project, this becomes labelled and listed (additionally) as [[MitoFit registered project]]. This is possible before (advance registration), during progress, and after completion of a study (post-registration).
-A [[MitoFit registered project]] receives a code for feeding data into the [[MitoFit data bank]].
-== Links to Publications ==
- [[MiPMap]]
-* [[Population]]
-# [[Property:Mammal and model]]
-# [[Property:Nonmammal]]
-# [[Property:Tissue and cell]]
-# [[Property:Cell line]]
-* [[Sample type]]
-:: = [[Population]] and Preparation
-# [[Property:Preparation]]
-# [[Property:Enzyme]]
-* Pathophysiological arms of an [[Experiment]]
-# [[Property:Stress]]
-# [[Property:Diseases]]
-* Respiratory control
-# [[Property:Respiration and regulation]]
-# [[Property:Coupling states]]
-# [[Property:Substrate states]]
-* Instrumental platform
-# [[Property:High-resolution respirometry]]
-== Links to MitoPedia ==
-* [[MitoPedia]]