Difference between revisions of "Talk:TMPD"

Latest revision as of 16:31, 18 October 2018

TMPD has been accessed more than

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-=== Complex IV assay ===
+[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]
-* [[Complex IV]] - TMPD concentration: 0.5 mM.
+== Popular Bioblast page ==
+[[TMPD]] has been accessed more than
-* It is important to add  [[Ascorbate]] before TMPD  to avoid uncontrollable autoxidation.  Ascorbate maintains TMPD in a  reduced state. Electrons flow from TMPD to cytochrome c.
+:*  10,000 times (2018-10-18)
+:*  5,000 times (2015-05-24)
-=== Preparation of 200 mM stock ===
-TMPD   (C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>*2HCl):  Sigma T 3134, 5 g, store at RT; FW = 237.2.
-::1) Weigh 47.4 mg TMPD
-::2) Dissolve in 1 ml H<sub>2</sub>O
-::3) Divide into 0.2 ml portions
-::4) Protect from light!
-::5) Store frozen at -20 °C
-<span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial). Take care to minimize light and temperature exposure during use!</span>
-'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 5 µl using a 25 µl syringe
-::* Final conc. in 2 ml O2k-chamber: 0.5 mM