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| === Complex IV assay === | | [[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]] |
| * [[Complex IV]] - TMPD concentration: 0.5 mM.
| | == Popular Bioblast page == |
| | | [[TMPD]] has been accessed more than |
| === Preparation of 200 mM stock ===
| | :* 10,000 times (2018-10-18) |
| TMPD (C<sub>10</sub>H<sub>16</sub>N<sub>2</sub>*2HCl): Sigma T 3134, 5 g, store at RT; FW = 237.2.
| | :* 5,000 times (2015-05-24) |
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| ::1) Weigh 47.4 mg TMPD
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| ::2) Dissolve in 1 ml H<sub>2</sub>O
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| ::3) Divide into 0.2 ml portions
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| ::4) Protect from light!
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| ::5) Store frozen at -20 °C
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| <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial). Take care to minimize light and temperature exposure during use!</span>
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| '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]]
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| ::* Titration volume: 5 µl using a 25 µl syringe
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| ::* Final conc. in 2 ml O2k-chamber: 0.5 mM
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Latest revision as of 16:31, 18 October 2018
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TMPD has been accessed more than
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