Talk:Zhang 2012 Nat Protoc
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Revision as of 07:10, 2 May 2022 by Gnaiger Erich (talk | contribs)
Discussion on limitations of this study:
- The authors use a ROCK inhibitor to prevent cells from clumping, but there is no information on whether a possible effect of this inhibitor on mitochondrial respiration has been tested.
- Short periods of high oxygen concentrations can influence stem cell properties. How did the authors transfer the stem cells from the incubator to the respirometer - was there a hypoxic atmosphere in the respirometer?
- The authors discuss the classical view on cell permeabilization and measurement of citrate synthase/lactate dehydrogenase, but do not mention the latest publications, e.g. up-to-date simple titration schemes.
- 50 Β΅M ADP was used. In isolated rat liver mitochondria the apparent Km is 52 Β΅M, corresponding to 50 % of OXPHOS capacity. The required ADP concentration needed to evaluate Jmax should be minimum 9 times the Km (Gnaiger 2001 Respir Physiol).