Template:Fluorescence dyes and settings
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Revision as of 15:25, 18 November 2021 by Komlodi Timea (talk | contribs)
Application | Sensor | Filter set | O2k output | Light intensity (Amp polarization voltage) | Gain | Comment |
---|---|---|---|---|---|---|
AmplexTM UltraRed | Fluorescence-Sensor Green | AmR | Type II | 100 - 500 | 1000 | at c(AmR) = 10 µM |
TMRM | Fluorescence-Sensor Green | AmR | Type III | 200 - 500 | 1000 | at c(TMRM) = 2 µM |
Safranin | Fluorescence-Sensor Blue | Saf | Type III | 100 - 200 | 1000 | at c(safranin) = 2 µM |
Magnesium Green™ | Fluorescence-Sensor Blue | MgG / CaG | Type I and II | 100 - 500 | 1000 | at c(Mg Green) = 2 µM |
Calcium Green™ | Fluorescence-Sensor Blue | MgG / CaG | Type I | 300 - 500 | 1000 | at c(Ca Green) = 2 µM |
In the O2k-LED2-Module (Series D-G) polarization voltage is used, while in the O2k-Fluo Smart Module (Series H-I) fluorescence intensity. To use the same setting in the O2k-Fluo LED2-Module as in the O2k-Fluo Smart-Module use the following Excel sheet:
Download the EXCEL calculation-sheet to adjust fluorescence intensity |