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::* Recommendation: Set "Slope smoothing" to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength [[resolution]], but increases the [[noise]] of the signal.
* Recommendation: Set "Slope smoothing" to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength [[resolution]], but increases the [[noise]] of the signal.
   
   
::: The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
:The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
[[File:Slope smoothing 40.png|700px|thumb|left|Slope smoothing 40]]
[[File:Slope smoothing 40.png|700px|thumb|left|Slope smoothing 40]]
[[File:Slope smoothing 20.png|700px|thumb|left|Slope smoothing 20]]
[[File:Slope smoothing 20.png|700px|thumb|left|Slope smoothing 20]]

Revision as of 11:31, 13 March 2019

  • Recommendation: Set "Slope smoothing" to 20 for performing and analyzing experiments with biological sample (strongly recommended in the case of uncoupler titrations). A slope smoothing of 20 increases the time or wavelength resolution, but increases the noise of the signal.
The following DatLab traces illustrate an example of "Slope smoothing" using 20 versus 40 data points:
Slope smoothing 40
Slope smoothing 20
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