Difference between revisions of "Template Select fluorescence sensor"
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:::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | :::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | ||
:::: Select the Fluo-Sensor and filter set from the table on [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores application-specific settings] (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set: | :::: Select the Fluo-Sensor and filter set from the table on [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores application-specific settings] (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set: | ||
::::# | ::::# [[Fluorescence-Sensor Green]] and [[Smart Fluo-Sensor Green]]: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H<sub>2</sub>O<sub>2</sub> measurement with [[Amplex red|Amplex® UltraRed]] or for measurements of mt-membrane potential with [[TMRM]]. | ||
::::# | ::::# [[Fluorescence-Sensor Blue]] and [[Smart Fluo-Sensor Blue]]: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]] or [[Rhodamine 123]]. A different filter is used for measurement with [[Magnesium green|Magnesium Green™]] or Calcium Green™. | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor] | |||
Latest revision as of 09:35, 19 November 2021
Select the Fluo-Sensors
- Switching between different excitation wavelengths and filters is achieved by simply exchanging the Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
- Select the Fluo-Sensor and filter set from the table on application-specific settings (here we should link to the respective section which is also a template). Each Fluo-Sensor is delivered with a mounted filter set:
- Fluorescence-Sensor Green and Smart Fluo-Sensor Green: 525 nm (dominant wavelength). The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed or for measurements of mt-membrane potential with TMRM.
- Fluorescence-Sensor Blue and Smart Fluo-Sensor Blue: 465 nm (dominant wavelength). The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin or Rhodamine 123. A different filter is used for measurement with Magnesium Green™ or Calcium Green™.
