Thiede 2012 Biochim Biophys Acta
| Thiede A, Gellerich FN, Schoenfeld P, Siemen D (2012) Complex effects of 17Ξ²-estradiol on mitochondrial function. Biochim Biophys Acta 1817:1747-53 |
Thiede A, Gellerich FN, Schoenfeld P, Siemen D (2012) Biochim Biophys Acta
Abstract: Existing literature on estradiol indicates that it affects mitochondrial functions at low micromolar concentrations. Particularly blockade of the permeability transition pore (PTP) or modulation of the enzymatic activity of one or more complexes of the respiratory chain were suspicious. We prepared mitoplasts from rat liver mitochondria (RLM) to study by single-channel patch-clamp techniques the PTP, and from rat astrocytes to study the potassium BK-channel said to modulate the PTP. Additionally, we measured respiration of intact RLM. After application of 17Ξ²-estradiol (Ξ²E) our single-channel results reveal a transient increase of activity of both, the BK-channel and the PTP followed by their powerful inhibition. Respiration measurements demonstrate inhibition of the Ca(2+)-induced permeability transition, as well, though only at higher concentrations (β₯30ΞΌM). At lower concentrations, we observed an increase of endogenous- and state 2-respiration. Furthermore, we show that Ξ²E diminishes the phosphorylating respiration supported by complex I-substrates (glutamate/malate) or by the complex II-substrate succinate. Taken together the results suggest that Ξ²E affects mitochondria by several modes, including partial inhibition of the activities of ion channels of the inner membrane and of respiration. β’ Keywords: Permeability transition pore (PTP), 17Ξ²-estradiol
β’ O2k-Network Lab: DE Magdeburg Gellerich FN, DE Magdeburg Siemen D, DE Magdeburg Schoenfeld P
Labels:
Organism: Rat
Tissue;cell: Nervous system, Liver
Preparation: Isolated mitochondria
Enzyme: Complex I, Complex II;succinate dehydrogenase
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
- This article is part of a Special Issue entitled: 17th European Bioenergetics Conference (EBEC 2012).
