Difference between revisions of "Tissue homogenate"
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Homogenized tissue provides | Homogenized tissue provides various advantages compared to isolated mitochondria or permeabilized fibres: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration ([[Pecinova 2011 Mitochondrion]]). | ||
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Disadvantages of Thom compared to isolated mitochondria include: (i) The presence of non-mitochondrial membranes increases nonspecific binding of indicators of mt-membrane potential. (ii) Cytosolic components of liver tissue interfere with the measurement of H2O2 production using Amplex red. (iii) Cytosolic components may increase [[ROX]]. |
Revision as of 13:01, 14 November 2013
Description
A tissue homogenate (Thom) is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized.
Abbreviation: Thom
Reference: MiPNet17.02 PBI-Shredder-Manual
MitoPedia methods:
Respirometry
Homogenized tissue provides various advantages compared to isolated mitochondria or permeabilized fibres: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration (Pecinova 2011 Mitochondrion).
Disadvantages of Thom compared to isolated mitochondria include: (i) The presence of non-mitochondrial membranes increases nonspecific binding of indicators of mt-membrane potential. (ii) Cytosolic components of liver tissue interfere with the measurement of H2O2 production using Amplex red. (iii) Cytosolic components may increase ROX.