Difference between revisions of "Tronstad 2012 Abstract Bioblast"
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|abstract=Mitochondria are major producers of ATP, which also make them important in processes of cellular adaptation, stress, and survival/death. In this sense they represent centers of crosstalk between metabolism and signaling. Depending on the cellular context, mitochondrial activities are determined by elements of metabolic/functional regulation, biomass and structural organization. Within cells, mitochondrial morphology varies from spherical individual organelles to interconnected filamentous networks. Mitochondrial architecture is controlled by fission and fusion processes, which are dynamic and regulated by intracellular conditions. Although evidence has been provided that mitochondrial shape and function are interconnected, more quantitative single cell studies are required. Furthermore, recent findings have given new insights into the roles of mitochondrial biogenesis in cellular responses to energy stress, mutations and aging. Clearly, the mechanisms directing mitochondrial biogenesis are diverse, and involve multiple key factors regulating crucial functions of the viable cell. In the present project, we are addressing the roles and mechanisms of mitochondrial morphology and biogenesis in cellular adaptation and stress tolerance. We will present a protocol allowing automated 3D analysis of mitochondrial number, volume, surface area, sphericity and network related descriptors (e.g. branching points) using z-stacks obtained by standard confocal microscopy. Furthermore, we will discuss data showing diverse cellular responses to “mitochondrial boosters”. The metabolic phenotype of a cell, including mitochondrial respiration, may represent an important determinant of stress tolerance. Using new tools to study these phenomena is therefore important in order to learn more about mitochondrial physiology. | |abstract=Mitochondria are major producers of ATP, which also make them important in processes of cellular adaptation, stress, and survival/death. In this sense they represent centers of crosstalk between metabolism and signaling. Depending on the cellular context, mitochondrial activities are determined by elements of metabolic/functional regulation, biomass and structural organization. Within cells, mitochondrial morphology varies from spherical individual organelles to interconnected filamentous networks. Mitochondrial architecture is controlled by fission and fusion processes, which are dynamic and regulated by intracellular conditions. Although evidence has been provided that mitochondrial shape and function are interconnected, more quantitative single cell studies are required. Furthermore, recent findings have given new insights into the roles of mitochondrial biogenesis in cellular responses to energy stress, mutations and aging. Clearly, the mechanisms directing mitochondrial biogenesis are diverse, and involve multiple key factors regulating crucial functions of the viable cell. In the present project, we are addressing the roles and mechanisms of mitochondrial morphology and biogenesis in cellular adaptation and stress tolerance. We will present a protocol allowing automated 3D analysis of mitochondrial number, volume, surface area, sphericity and network related descriptors (e.g. branching points) using z-stacks obtained by standard confocal microscopy. Furthermore, we will discuss data showing diverse cellular responses to “mitochondrial boosters”. The metabolic phenotype of a cell, including mitochondrial respiration, may represent an important determinant of stress tolerance. Using new tools to study these phenomena is therefore important in order to learn more about mitochondrial physiology. | ||
|keywords=Fission, Fusion, Morphology | |||
|mipnetlab=NO Bergen Tronstad KJ, | |mipnetlab=NO Bergen Tronstad KJ, | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|articletype=Abstract | |articletype=Abstract | ||
}} | }} | ||
{{Labeling|journal=Mitochondr Physiol Network | {{Labeling | ||
|injuries=Cancer; Apoptosis; Cytochrome c, Mitochondrial Disease; Degenerative Disease and Defect, Aging; Senescence | |||
|topics=Mitochondrial Biogenesis; Mitochondrial Density | |||
|journal=Mitochondr Physiol Network | |||
|articletype=Abstract | |articletype=Abstract | ||
}} | }} | ||
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== Affiliations and author contributions == | == Affiliations and author contributions == | ||
University of Bergen, Department of Biomedicine, Norway; Email: karl.tronstad@biomed.uib.no | |||
Revision as of 19:08, 18 November 2012
| Tronstad KJ (2012) Mitochondrial morphology and biogenesis in cellular stress. Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast 2012 - Open Access
Tronstad KJ (2012)
Event: Bioblast 2012
Mitochondria are major producers of ATP, which also make them important in processes of cellular adaptation, stress, and survival/death. In this sense they represent centers of crosstalk between metabolism and signaling. Depending on the cellular context, mitochondrial activities are determined by elements of metabolic/functional regulation, biomass and structural organization. Within cells, mitochondrial morphology varies from spherical individual organelles to interconnected filamentous networks. Mitochondrial architecture is controlled by fission and fusion processes, which are dynamic and regulated by intracellular conditions. Although evidence has been provided that mitochondrial shape and function are interconnected, more quantitative single cell studies are required. Furthermore, recent findings have given new insights into the roles of mitochondrial biogenesis in cellular responses to energy stress, mutations and aging. Clearly, the mechanisms directing mitochondrial biogenesis are diverse, and involve multiple key factors regulating crucial functions of the viable cell. In the present project, we are addressing the roles and mechanisms of mitochondrial morphology and biogenesis in cellular adaptation and stress tolerance. We will present a protocol allowing automated 3D analysis of mitochondrial number, volume, surface area, sphericity and network related descriptors (e.g. branching points) using z-stacks obtained by standard confocal microscopy. Furthermore, we will discuss data showing diverse cellular responses to “mitochondrial boosters”. The metabolic phenotype of a cell, including mitochondrial respiration, may represent an important determinant of stress tolerance. Using new tools to study these phenomena is therefore important in order to learn more about mitochondrial physiology.
• Keywords: Fission, Fusion, Morphology
• O2k-Network Lab: NO Bergen Tronstad KJ
Labels:
Stress:Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., Mitochondrial Disease; Degenerative Disease and Defect"Mitochondrial Disease; Degenerative Disease and Defect" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., Aging; Senescence"Aging; Senescence" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property.
Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Affiliations and author contributions
University of Bergen, Department of Biomedicine, Norway; Email: karl.tronstad@biomed.uib.no
